ampholyte


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ampholyte

(ˈæmfəˌlaɪt)
n
an amphoteric electrolyte
Translations
ampholyte
References in periodicals archive ?
Cy2, Cy3, Cy5, Deep Purple, pH 3-10 NL IPG strip (24cm),1,4-dithiothreitol (DTT), Acrylamide (Acr), carrier ampholyte, lysine, sodium dodecyl sulfate (SDS), N,N'-methylenebisacrylamide (Bis), 2D-Quant protein assay kit, thiourea and urea were obtained from GE Healthcare (US).
Cell pellets were lysed in 7 M urea, 2 M thiourea, 4% w/v CHAPS, 65 mM DTT, 2% v/v of Biolyte 3/10 Ampholyte 40% (Bio-Rad, Hercules, CA, USA), 1% v/v protease inhibitor cocktail (GE Healthcare, USA) and 1% v/v nuclease mix (GE Healthcare, USA) at room temperature for 1 h with occasional vortexing.
5 [micro]L of a solution containing 150 [micro]g of protein, 40 mM DTT, 2% ampholyte (IPG Buffer pH3-10) and Destreak rehydration solution (GE Healthcare).
we can assert that synthesized polymeric ampholyte contains in the structure except amino groups also phosphate groups.
2% Ampholyte, 10 mM DTT for 16 hr and then loaded onto 17 cm immobilized (pH=3-10) nonlinear gradient strips (Bio-Rad, Hercules, CA, USA).
Two-dimensional protein electrophoresis was undertaken using constant protein loading as previously described (5,6) using an ampholyte pH range 3.
01[micro]M ortho phosphoric acid as anolyte, 8M urea and 250[micro]l of ampholyte as sample overlay.
2% pH 3-10 linear IPG Ampholyte (Bio-Rad Laboratories, USA), 4% CHAPS (Sigma, USA), 1% HED (2-hydroxyethyldisulfide, Sigma, USA), and 1% DTT (Dithiothreitol, Sigma, USA).