ampholyte


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ampholyte

(ˈæmfəˌlaɪt)
n
an amphoteric electrolyte
Translations
ampholyte
References in periodicals archive ?
The ability to freeze and store cells for cell-based therapies and research has taken a step forward in the paper 'A synthetically scalable poly(ampholyte) which dramatically Enhances Cellular Cryopreservation.' published by the University of Warwick's Department of Chemistry and Medical School in the journal Biomacromolecules.
The protein pellet obtained from the 2D Clean-Up kit was resuspended in a lysis buffer containing 7M urea, 2M thiourea, 4% CHAPS, 40mM Tris, 120mM dithiothreitol (DTT), 2% ampholyte pH 3-10, and 1% protease inhibitor mix.
Gaigneaux, "Supramolecular organization in organic-inorganic heterogeneous hybrid catalysts formed from polyoxometalate and poly(ampholyte) polymer," Langmuir, vol.
Cy2, Cy3, Cy5, Deep Purple, pH 3-10 NL IPG strip (24cm),1,4-dithiothreitol (DTT), Acrylamide (Acr), carrier ampholyte, lysine, sodium dodecyl sulfate (SDS), N,N'-methylenebisacrylamide (Bis), 2D-Quant protein assay kit, thiourea and urea were obtained from GE Healthcare (US).
Cell pellets were lysed in 7 M urea, 2 M thiourea, 4% w/v CHAPS, 65 mM DTT, 2% v/v of Biolyte 3/10 Ampholyte 40% (Bio-Rad, Hercules, CA, USA), 1% v/v protease inhibitor cocktail (GE Healthcare, USA) and 1% v/v nuclease mix (GE Healthcare, USA) at room temperature for 1 h with occasional vortexing.
The IgG solution was then brought to dryness in SpeedVac and dissolved in 5.0 mM Trizma base, 9.0 M urea, 2% (w/v) CHAPS, and 0.2% (w/v) ampholyte, with pH range 3-10.
The protein pellets were re-solubilized in IPG rehydration buffer (7M urea 2M thiourea 2% CHAPS 50mM DTT 0.2% Bio-Lyte ampholyte 0.001% bromophenol blue) and then centrifuged.
The total proteins were dissolved in IPG rehydration/sample buffer (8 M urea, 2% CHAPS, 50 mM DTT, 0.2% Bio-Lyte 4/7 ampholyte, 0.001% Bromophenol Blue; Bio-Rad) and centrifuged at 12,000 g for 15 min at room temperature to remove nondissolved materials.
For the extraction of protein, 5 [micro]M ALN treated and untreated (control) cells were lysed in lysis buffer containing 8 M urea, 0.2 M EDTA, 0.5 M DTT, glycerol, NP40, ampholyte solution pH 3-10 in 0.5 M Tris-HCl with protease inhibitor cocktail.
we can assert that synthesized polymeric ampholyte contains in the structure except amino groups also phosphate groups.
One hundred and twenty [micro]g from each sample was resuspended in rehydration buffer containing 8 M urea, 4% CHAPS, 2 mM TBP (tributyl phosphate), 0.2% Ampholyte, 10 mM DTT for 16 hr and then loaded onto 17 cm immobilized (pH=3-10) nonlinear gradient strips (Bio-Rad, Hercules, CA, USA).