A 57-amino acid pea chloroplast transit peptide was fused to the N-terminus of the sequence to target protein expression to the amyloplast
, a nonpigmented organelle responsible for starch synthesis and storage.
Starch is found in amyloplast
in granules form and accounts for 65-70% of grain (Li et al., 1999).
Starch staining of the hypocotyls with 1-KI solution shows absence of amyloplast
granules in suppressors grown on sucrose free medium.
It occurs as granules in the chloroplast of green leaves and the amyloplast
of seeds, pulses, and tubers.
A statolith is an object in the plant cell now thought to be an amyloplast
, which belongs to a class of colorless plastids called leucoplasts.
The most noticeable correlated event we have noted is the asynchronous appearance of amyloplast
in microspores in the loculus.
The translocator protein is like a door that allows glucose to move out of the amyloplast
. Scientists want to shut that door, and this mutation does that.
Wasserman of Rutgers University, whose work focuses on the synthesis of amylose and amylopectin, and the molecular biology of the enzymes that catalyze chain elongation, insertion of branch points and spatial distribution of enzymes within the corn endosperms amyloplast
Altered gravitropic response, amyloplast
sedimentation and circumutation in the Arabidopsis shoot gravitropism 5 mutant are associated with reduced starch levels.
Starch staining of the hypocotyl with I-KI solution shows almost an absence of amyloplast
granules in the suppressors grown in sucrose free medium.
In addition, at 4[degrees]C amyloplasts
sedimented normally in the gps mutants, indicating that the GPS loci affect an aspect of the gravity signal perception/transduction pathway that occurs after amyloplast
They suggested that this inverse relationship may be due to differences in amyloplast
membranes, such as the composition of membrane lipids.