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Only the blood samples for glucagon analyses were collected in chilled lavender-top tubes containing 100 [micro]L of aprotinin (Sigma, Aprotinin, Cat# A6279).
The samples were also subjected to the CCm-Tf films impregnated with a mixture of aprotinin and elastinal or a mixture of aprotinin, elastinal, and 1,10-phenanthroline.
In preclinical testing, Textilinin has compared favourably to other antifibrinolytic products such as Trasylol (aprotinin), a product used to reduce perioperative blood loss in patients undergoing coronary artery bypass graft surgery (CABG).
The section on aprotinin was clearly written before the recent data on its associated morbidity and mortality was available; it has since been withdrawn from the market.
After the treatment, cell lysates were prepared using lysis buffer (1% (v/v) Triton X-100, 10 mM Tris base pH 7.6, 5 mM EDTA, 50 mM sodium chloride, 30 mM sodium pyrophosphate, 50 mM sodium fluoride, 0.1% (w/v) sodium azide, 50 mM phenyl methyl sulfonyl fluoride, 0.5 mg/mL aprotinin, 2.5 mg/mL leupeptin, and 100 mM sodium orthovandate in distilled water, pH 7.6).
Fifteen of the trials tested a multitude of other injectates including the sclerosing solution lauromacrogol, platelet-rich plasma, sodium hyaluronate, prolotherapy (solution of hypertonic glucose and local anaesthetic), aprotinin, and botulinum toxin.
Klaus M, Wiesner G, Breuer T, Lange R, Tassani P 2008 The risks of aprotinin and tranexamic acid in cardiac surgery; a one-year follow-up of 1188 consecutive patients Anesthesia and Analgesia 107 (6) 1783-1790
PaTS was the least inhibited by big (SBTI) and small (aprotinin) proteinaceous inhibitors, while it was the most affected by small synthetic inhibitors (PMSF, TLCK) (Table 2), suggesting different structural features.
There is neither scientific rationale for benefit nor experience with systemic hemostatics (desmopressin and aprotinin) in individuals receiving rivaroxaban.
(22) However, a recent observational study involving 4,374 patients undergoing CABG indicated that the use of aprotinin was associated with a doubling in the risk of renal failure requiring dialysis, a 55 percent increase in the risk of myocardial infarction or heart failure, and a 18.1 percent increase in the risk of stroke or encephalopathy.