Kaizer et al., "Apyrase
and 5'-nucleotidase activities in synaptosomes from the cerebral cortex of rats experimentally demyelinated with ethidium bromide and treated with interferon-[beta]," Neurochemical Research, vol.
APT Therapeutics has applied proprietary protein engineering strategies to produce APT102, an innovative human apyrase
therapy intended for the treatment of thrombotic diseases.
Shih, "Signal-amplification and real-time fluorescence anisotropy detection of apyrase
by carbon nanoparticle," Materials Science and Engineering C, vol.
In this experimental setting, we added apyrase
(0.5 U/ml), an enzyme that efficiently cleaves endogenous ATP that might be released by LPS-primed and nigericin-treated U937 cells.
Washed platelets (WPs) were obtained from platelet-rich plasma (PRP), isolated following centrifugation at 100g for 10 min of citrated blood as previously described , with serial centrifugation and addition of 0.2 mM [PGI.sub.2] and 0.01 mg/l apyrase
. Platelet pellets were resuspended in HEPES-Tyrode's buffer (137mM NaCl, 20 mM HEPES, 5.6 mM glucose, 0.35% bovine serum, 1 mM Mg[Cl.sub.2], 2.7 mM KCl, and 3.3 mM Na[H.sub.2]P[O.sub.4]).
In this biosynthetic pathway, dihydroorotase that converts glutamine to dihydroorotate, dihydroorotate dehydrogenase that converts dihydroorotate to orotic acid, uridine monophosphate synthetase that converts orotic acid to UMP, 5,-nucleotidase that converts UMP to uridine, uridine nucleosidase that converts uridine to uracil, uridine kinase (udk) that converts cytidine to CMP, uridine/cytidine monophosphate (UMP-CMP) kinase that converts CMP to CDP, nucleoside-diphosphate kinase (ndk) that converts UDP to UTP, apyrase
that converts CTP to CDP, inosine triphosphate pyrophosphatase (ITPA) that converts UMP to UTP, CTP synthase that converts UTP to CTP, and cytidine deaminase (cdd) that converts cytidine to uridine were annotated in the H.
This pyrene-based sensor was used in HeLa cells to monitor the decrease in ATP levels upon addition of the ATP synthase inhibitor, oligomycin, and upon hydrolysis of ATP to ADP by apyrase
(Pak et al., 2015).
SmATPDasel, but not SmATPDase2, hydrolyses exogenous ATP and ADR Purinergic Signal.
This increase was prevented by the NFAT blocker A-285222 (1 [micro]mol) and also by the ectonucleotidase apyrase
(3.6 U/mL), implicating extracellular nucleotides in the activation of NFATc3 (Supplementary Figures 2(a)-2(b)).
, serpin 1 and 2, D7 protein, adenosine deaminase (ADA), a serine protease, amylase, actin, purine nucleosidase, lectin (Ribeiro et al.
The platelet pellets were suspended in 5 mL of Tyrode's solution, pH 7.3 [containing (mM) NaCl 11.9, KCl 2.7, Mg[Cl.sub.2] 2.1, Na[H.sub.2]P[O.sub.4] 0.4, NaHC[O.sub.3] 11.9, and glucose 11.1]; then apyrase
(1.0U/mL), [PGE.sub.1] (0.5 [micro]M), and heparin (6.4 IU/mL) were added, and the mixture was incubated for 10 min at 37[degrees]C.
Furthermore, leech saliva is an effective platelet aggregation inhibitor due to the presence of active ingredients such as calin, apyrase
, platelet activating factor (PAF-) antagonist, collagenase, and prostaglandin.