Ninety prostate cancer specimens were used to construct a tissue microarray by manual tissue arrayer
. Representative areas of each paraffin block were labelled by B.K.
Urinary proteins were spotted on the surface of an epoxy modified glass slide using a Smart Arrayer
48 (Capital Bio Corp.
The TMA was constructed with 1-mm-diameter cores using a manual tissue arrayer
(Beecher Instruments, Silver Spring, MD, USA) as described previously .
Tissue microarrays (TMAs) were constructed using a manual tissue arrayer
purchased from Beecher Instruments (Woodland, WI, USA) as previously described .
Every patient's tumor tissue on the TMA was consecutive, and the TMA was constructed using a tissue arrayer
with 5 fim thickness.
El tissue arrayer
consta de dos agujas de distintos calibre con las que se realiza el troqueleado de los bloques.
(13) A Beecher Instruments Manual Tissue Arrayer
(Sun Prairie, Wisconsin) was used to prepare tissue cores from selected regions of archival tissue blocks.
Tissue microarray (TMA) of 1.0 mm core size was constructed as described previously  using a semiautomatic Tissue Arrayer
Minicore (Alphelys, SAS, France).
Each capture antibody diluted to 100 [micro]g/mL in PBS-Trehalose (Dulbeccos's phosphate buffered saline containing 50 mM D-(+) Trehalose) was loaded onto a 384-well plate (Genetix) and printed in in four sets of triplicate spots in a 16 x 16 array format onto a poly-l-lysine-coated glass slide (Thermofisher, UK) using a Biorobotics Microgrid II arrayer
(Microgrid) and a silicon contact pin (Parallel Synthesis Technologies, USA).
From each carcinoma, two tissue cores (1 mm in diameter) were obtained from the main tumor mass and two cores were taken from the tumor at the positive surgical margin; these samples were arrayed in a recipient paraffin block using MTA Booster OI Manual Tissue Arrayer
(Alphalyse, Plaisir, France).
Pure tumor areas from the primary Gleason grade were punched with use of the manual tissue arrayer
(Beecher Instruments Tissue Microarray Technologies), creating cores 0.6 mm in diameter and 5 mm in length.