The major autolysin
of Staphylococcus lugdunensis, AtlL, is involved in cell separation, stress-induced autolysis and contributes to bacterial pathogenesis.
Bacterial DNA is released by the action of the secreted metalloprotease gelatinase (GelE) on the major autolysin
(AtlA), but only in a subpopulation of cells (ca 15%) that do not, concomitantly, express serine protease (SprE) for preventing autolysis.
aureus cell lysis by inhibiting the activity of the enzyme autolysin
as well as maintaining the rigidity of the CW .
Beveridge, "A major autolysin
of Pseudomonas aeruginosa: Subcellular distribution, potential role in cell growth and division, and secretion in surface membrane vesicles," Journal of Bacteriology, vol.
Jansen et al., "Enterococcus faecium biofilm formation: identification of major autolysin
AtlAEfm, associated acm surface localization, and AtlAEfm-independent extracellular DNA release," mBio, vol.
Streptococcus pneumoniae was detected by lytA real-time PCR (selective for autolysin
gene), and serotyping was performed by a nanofluidic real-time PCR that detects 50 serotypes as individual serotype/serogroup including all vaccine serotypes (6).
Nowadays efforts are being made to investigate virulence genes coding for pneumolysin (ply) and autolysin
(lytA), as these two antigens have been linked up with virulence of S.
In pneumococcal cultures the products of the metabolism and the activation of the autolysin
could cause a decrease of the amount of cells and the loss of viability .
Essential role for the major autolysin
in the fibronectin-binding protein-mediated Staphylococcus aureus biofilm phenotype.
Characterization of the importance of Staphylococcus epidermidis autolysin
and polysaccharide intercellular adhesin in the pathogenesis of intravascular catheter-associated infection in a rat model.
TAs can also help in creating a pH gradient across the cell wall and are also known to be involved in phage adsorption and autolysin
This assay utilized the autolysin
gene and was evaluated using only a small number of culture-negative clinical samples.