The mRNA products were purified and resolved on 7% denaturing polyacrylamide gel, followed by autoradiogram
. Transcription (%) was set to 100% as to the fold activation (signal from GAL4 template divided by signal from GCN4 template) shown by wild-type holoenzyme upon PIC formation at 37[degrees]C.
Genotyping analysis: Each allele appeared has a peak on electropherogram and as band on autoradiogram
like tiff image file generated by Licor 4300 Genetic Analyzer.
The developed film, called an autoradiogram
, shows the pattern of a DNA profile.
showed a white band on a black background that corresponded to the sphingomyelinase activity.
A melanoma-bearing mouse showed, on the whole body autoradiogram
, the selective uptake and covalent binding of NAcCAP in melanoma tissues of lung and skin .
Students are reminded that nucleic acid is not normally visible on the filter at this point, but upon adding a probe that is, for example, specific for the molecule represented by the red dye, that molecule will be detected on an autoradiogram
. These final steps (i.e., probe hybridization through film exposure) are illustrated in the remaining slides of my PowerPoint presentation.
Historically, the standard approach to measuring mtDNA content is Southern blot analysis with radioactive probes for both mtDNA and nuclear DNA, followed by densitometric analysis of the autoradiogram
or phosphoimage (18).
The DNA was transferred from the gel to a nylon Hybond [N.sup.+] membrane (Amersham, Piscataway, NJ) by capillary blotting, prehybridized with sheared salmon sperm DNA, hybridized overnight with [.sup32p]dCTP-labeled Cp3-13 probe, and viewed by autoradiogram
Make also a drawing of the autoradiogram
for control mice and transgenic mice that contain two copies of the transgene.
Restriction sites were inferred by examination of autoradiogram
banding patterns from adjacent cpDNA clones.
"Classically, people use restriction enzyme digests (to characterize the DNA), they hold a piece of film up on an autoradiogram
and look for common patterns.
Whole Body Autoradiogram
. At the time of single oral administration of [sup.14]C-hyaluronic acid at a dose of 25 mg/kg in male SD rats aged 7 to 8 weeks, [sup.14]C was detected in the skin as follows: 2.36 PSL/[mm.sup.2] at 8 hours, 3.81 PSL/[mm.sup.2] at 24 hours, and 1.98 PSL/[mm.sup.2] at 96 hours after administration.