avidin


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av·i·din

 (ăv′ĭ-dĭn)
n.
A protein found in uncooked egg white that binds to and inactivates biotin.

[avid + (biot)in, from its affinity for biotin.]

avidin

(ˈævɪdɪn; əˈvɪdɪn)
n
(Biochemistry) a protein, found in egg-white, that combines with biotin to form a stable compound that cannot be absorbed, leading to a biotin deficiency in the consumer
[C20: from avid + (bio)in; from its characteristic avidity for biotin]

av•i•din

(ˈæv ɪ dɪn, əˈvɪd ɪn)

n.
a protein of raw egg white that combines with the vitamin biotin and prevents its absorption.
[1940–45; avid + (biot) in; so named from its affinity for biotin]
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References in periodicals archive ?
Slides were then incubated in ABC (avidin biotin) reagent and washed in TBS buffer again for 2-5 minutes.
Cross-linker biotin binding proteins such as Avidin, Streptavidin, and NeutrAvidin can be attached to the biotinylated PLGA NP.
The iminobiotin coupled with anticancer drugs shows a high affinity to avidin (and avidin-immobilized magnetic nanoparticles (AMNPs)) at elevated pH (9.5-10.8) but releases the drugs easily at lower pH (~4) or under the presence of a certain concentration of biotin at pH 6.8 according to the manufacturer's instructions.
When ABC-HRP was used, endogenous biotin reactivity was blocked by supplementing the 5% goat serum (Sigma) during blocking with 1: 20 avidin and the primary antibody staining solution with 1 : 20 biotin (Avidin-biotin blocking kit; Vector Labs), with 3 x 2min washes with PBS in between.
Then, sections were blocked with 10% normal serum from the species of secondary antibody, Avidin D solution (Avidin/Biotin Blocking Kit, Vector, Burlingame, CA, USA) and 0.25% Triton X-100 for 30 min.
(6) The biotin molecule, which binds irreversibly to avidin, can be conjugated to an immunoglobulin molecule by a straightforward amino substitution at an alkaline pH with no loss of immunoglobulin activity.
EW also contain avidin. Avidin complexes with biotin to impair its digestion [27], which can lead to a biotin deficiency if consumed in large amounts that exceed the capacity of pepsin [20, 28].
The PCR solution and carbon particles conjugated with avidin were directly added to the nitrocellulose membrane with two test lines of immobilized antibodies specific for digoxigenin and fluorescein.
(53) The size of avidin with the molecular mass of 70 kDa is ~5 nm in diameter, which is large enough to be visualized directly by electron microscopy.
Comparison of avidin biotin ELISA with RBPT and STAT for screening of antibodies to bovine brucellosis.