bacterial artificial chromosome


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Related to bacterial artificial chromosome: Yeast artificial chromosome

bacterial artificial chromosome

n. Abbr. BAC
A large DNA sequence that is artificially inserted into a bacterial genome for replication or expression.
References in periodicals archive ?
The global multiplexed molecular diagnostics market segmentation is based on technology (low density multiplexed assays, microplate assays, quantitative PCR, medium density multiplexed assays, antibody arrays, bead-based arrays, mass spectrometry, reverse arrays, high density multiplexed assays, bacterial artificial chromosome arrays, protein arrays, very high density multiplexed assays, oligonucleotide arrays, phage display, next generation sequencing assays), and applications (allergies, autoimmune diseases, cardiac disorders, infectious disease diagnosis, oncology).
Construction and preliminary characterization of a river buffalo bacterial artificial chromosome library.
Abbreviations: BAC, bacterial artificial chromosomes; BAC-FISH, fluo-rescently labeled bacterial artificial chromosome in situ hybridization; DAPI, 4'-6-diamidino-2-phenylindole; FISH, fluorescent in situ hybridization; FSW, filtered seawater; PABA.
To begin with, chromosomal DNA was cut by certain enzymes to produce overlapping fragments (100,000--200,000 base pairs long), each of which was inserted into a DNA piece called a bacterial artificial chromosome (BAC).
The contract includes gene discovery and BAC (bacterial artificial chromosome) screening across the Interleukin-1 (IL-1) region.
The global multiplexed diagnostics market segmentation is based on technology (low density multiplexed assays, microplate assays, quantitative PCR, medium density multiplexed assays, antibody arrays, bead-based arrays, mass spectrometry, reverse arrays, high density multiplexed assays, bacterial artificial chromosome arrays, protein arrays, very high density multiplexed assays, oligonucleotide arrays, phage display, next generation sequencing assays), and applications (allergies, autoimmune diseases, cardiac disorders, infectious disease diagnosis, oncology).
A number of resistant genes have been mapped and isolated by cloning strategy in the last decade in rice and other cereal crops (Eitas and Dangl,2010).The cloning of single QTL of the interest is now possible by merging marker technology with genomic resources such as bacterial artificial chromosome (BAC), yeast artificial chromosome (YAC), linkage map construction and by exploiting developed plant material (Paran and Zamir, 2003; Salvi and Tuberosa, 2005).QTL and its linked markers associated with blast disease in rice have been mapped by various researchers (Ashkani et al., 2013a; Sharma et al., 2005).
In addition, Hehir-Kwa et al (11) assessed 4 genome-wide copy number profiling methods for their ability to detect submicroscopic single-copy number variations, and they determined that commercially available aCGH and SNP microarray platforms performed better than an in-house tiling resolution bacterial artificial chromosome array of 32 447 overlapping bacterial artificial chromosome clones.
This whole-genome array of 2600 bacterial artificial chromosome (BAC) clones (Spectral Genomics, Inc.) spaced 1 Mb apart showed a gain in copy number of BAC clones extending from clone RP11-1K11 at 8p23.2 (chr8: 4 596 114-4 755 793; human genome build 18) to clone RP11-23H1 at 8p22 (chr8: 15 027 287-15 191603; hg18) indicative of an approximately 10.6-Mb duplication at 8p22p23.2.
In a unique application, a bacterial artificial chromosome (BAC) fingerprinting technique was adapted for high-throughput analysis by Sylvie Quiniou, a CGRU molecular biologist, in conjunction with MSAGL.
Efficient cloning of plant genomes into bacterial artificial chromosome (BAC) libraries with larger and more uniform insert size.