However, expression of either VP 1 or both VP 1 and VP2 with recombinant baculoviruses
formed viruslike particles that are morphologically and antigenically similar to the native virion (2).
The virus, known as AcMNPV, belongs to a group of microorganisms called baculoviruses
. Worldwide, says Vail, scientists have found hundreds of insect-killing baculoviruses
In order to obtain large amounts of gp 160 material, the researchers are growing genetically engineered baculoviruses
in cultures containing insect cells.
have limitations, which are restricting their use for insect control in practice.
New cell lines from Lymantria xylina (Lepidoptera: Lymantriidae): characterization and susceptibility to baculoviruses
Genetic variation in field populations of baculoviruses
: mechanisms for generating variation and its potential role in baculovirus epizootiology.
As shown in Figure 2(a) (left panel), baculoviruses
were detected by gp64 antibody staining; however, UVADEX treatment inactivates baculoviruses
to express gp64 (Figure 2(a), right panel).
Preparation of Recombinant Baculoviruses
. A1 [micro]g quantity of the recombinant bacmids and 6 [micro]L of Cellfectin II Reagent were separately diluted using 100 [micro]L of incomplete Grace's medium (without antibiotics and FBS).
Unlike most lepidopteran baculoviruses
, Hymenoptera baculovirus can only replicate in the epithelial cells in the midgut of larvae.
The recombinant baculoviruses
carrying the genes for the viral capsid proteins of the lion/NoV/GIV.2/Pistoia/387/06/ITA and Hu/NoV/GIV.l/SaintCloud624/1998/ U.S.
Jehle JA, Blissard GW, Bonning BC, Cory JS, Herniou EA, Rohrmann GF, Theilmann DA, Thiem SM, Vlack JM (2006) On the classification and nomenclature of baculoviruses
: A proposal for revision.