For effective results, various stains (lactophenol and cotton blue, Melzer's reagent, congo red, etc.) were used to visualize different cellular structures such as hyphae, basidia
, and spores.
23-33 x 6.5-7.5 [mu]m, clavate, tetrasporic, rarely mono- or bisporic, hyaline (Fig.
clavate, 4 spored, brown in KOH, 31.6-35.1 x 6.8-10 um.
Measurements for basidia
, basidiospores and cheilocystidia were made using an ocular micrometer.
is a large and heterogeneous assemblage of species in Agaricomycetes, kept together on the basis of generally large sized, smooth basidiospores and constricted basidia
, rich in both oil drops and hyphae with conspicuous clamps.
were cylindrical 42 x 4 [micro]m in size with 3 transverse septa.
Taxonomy and phylogeny of yellow Clavaria species with clamped basidia
- Flavostellifera sp.
Also, the similar microscopic features, notably spore and basidia
shape and size, adds weight to the conclusion that the two species may be part of a complex (Table 1).
(13-)(15-)(20-)22-29 x (4-) (5-) 6-7 [micro]m, 4-spored, clavate or subvesiculose-subcylindric, with a middle constriction, hyaline.
Bandoni (1984) proposed an alternative classification of Tremellales and Auriculariales based on the characteristics of the basidia
, the haploid stage of mycelia and the septal pore apparatus; however theses characteristics have not been used to differentiate species.
Segundo BEDENDO (1995), os basidiosporos sao ovalados, unicelulares, uninucleados, hialinos, normalmente nao existem ornamentacoes e sao produzidos a partir da germinacao dos teliosporos, os quais dao origem a um pro-micelio (tubo germinativo) que se diferencia numa basidia
. No caso da familia Tilletiaceae, a basidia
e asseptada, e os basidiosporos sao formados em tufos, situados na extremidade da basidia
18-26 x (5.5-) 6-7 (-8) [micro]m, 4-spores, sterigmata 4-5 x 1-1.5 [micron]m, clavate-ventricose, sometimes with a middle constriction, some basidia
produced spores type 1 and others type 2.