Peroxidase activities were measured with 3,3',5,5'-tetra methyl benzidine
(TMB) as the reductive substrate (JOHNSEN & JACOBSEN, 2008).
After these procedures, color reaction with tetramethyl benzidine
was quantified in a plate reader at 450 nm (Quick Elisa, Thermo Fisher Scientific, USA).
Mutagenicity of benzidine
and benzidine-congener dyes and selected monoazo dyes in a modified Salmonella assay.
Bone marrow haematopoietic cell fate identification applying Graham-Knoll Benzidine
staining also needs to be done to see the effect in all blood cells morphologies and stages.
After, buffer washing, 100 ul of Tetra Methyl Benzidine
(TMB) substrate was added and incubated for 15 minutes at room temperature.
Slides were incubated in Diamino benzidine
(DAB) or other suitable peroxidase substrate.
Peroxidase (PER) activity was detected by spectrophotometry at 600 nm using benzidine
reagent (Litvin, 1981).
Exposure to certain industrial chemicals, such as aniline dyes, benzidine
and xenylamine, is also thought to be linked to a higher rates of bladder cancer.
Peroxidase activity was revealed by adding 100 [micro]l per well of tetra methyl benzidine
(Sigma-Aldrich) in dark.
The slides were developed using a diamino- benzidine
(DAB)-peroxidase substrate for 20-30 min at room temperature.
Controlling the FDR at 0.1, notable positive associations were for 1,4-dioxane, bromoform, dibenzofurans, glycol ethers, methyl ferf-butyl ether (MTBE), and propionaldehyde; whereas 1,4- dichlorobenzene, 4,4'-methylene diphenyl diisocyanate (MDI), benzidine
, and ethyl carbamate (urethane) were inversely associated with an ASD diagnosis.