Dark urine has been associated with hemoglobinuria [10], myoglobinuria [10], hematuria [7], and bilirubinuria [7, 11], all indicators of liver or kidney dysfunction [7, 12].
(3) Some of the key clinical features of cholestasis of infancy are jaundice persisting beyond the age of 14 days, jaundice with elevated serum conjugated bilirubin fraction (>2.0 mg/dl or >20% of total bilirubin), variably acholic stool, dark urine that stain the diaper yellow, bilirubinuria, and hepatomegaly.
However, none of our patients had viral hepatitis and the increased bilirubinuria and uro bilinogenuria observed in the present study cannot be attributed to viral co-infection.
In dogs with acute hepatitis, the decrease of the bilirubinaemia to values of 0.85-1.1 mg/dl following apitherapy as compared to 1.9-2.2 mg/dl in medicinal treatment, correlated with the decrease of the ALT activity (36-68 U/l) following the nutritional therapy with the liver protecting product as compared to values of 88-109 U/l following the medicinal treatment, justify the use of apitherapy showing the tendancy to recover, proved also by the normalization of the albuminaemia, bilirubinuria and the activities of PAL and yGT.