References in periodicals archive ?
Sequencing and library construction of approximately 150 DNA samples for whole genome sequencing (WGS), 25 samples for whole genome methylome/ bisulfite sequencing (WGBS), 30 samples of whole exome sequencing (WES), and 150 RNA samples for stranded total RNA seq (totalRNAseq) and 50 RNA samples from cell lines using polyA-RNA quantification (polyA-RNA).
Based on bisulfite conversion, oligonucleotides of methylated or nonmethylated probes and targets were synthesized for the DNA methylation assay.
RADICAVA contains sodium bisulfite, a sulfite that may cause a type of allergic reaction that can be serious and life-threatening.
Genome-wide paired-end bisulfite sequencing was applied to plasma DNA samples (4,5).
Real-time polymerase chain reaction (RT-PCR) and bisulfite genomic sequencing show a correlation between the state of DNA methylation within the promoter or exon1 CpG-islands and the levels of Pax7 and MRFs expression in cell myogenic differentiation.
Single-cell genome-wide bisulfite sequencing for assessing epigenetic heterogeneity"; Nature Methods, 20 July 2014 DOI: 10.
4-t-butyl cyclohexanone, piperidine, paraformaldehyde, acetic acid, hydroxyl amine hydrochloride, sodium acetate, sodium bisulfite, potassium cyanide, diethyl ether and other chemicals were purchased from Merck chemical Co.
Bisulfite treatment and polymerase chain reaction (PCR) amplification.
Supply of 875 tons sodium mita bisulfite of food grade & of EU origin.
Techniques based on DNA modification by sodium bisulfite are unable to distinguish between methylation and hydroxymethylation, since both 5-mC and 5-hmC do not undergo deamination after reacting with sodium bisulfite, and 5-mC and 5-hmC contents are mixed up in the final results.
Highlights of Hanks' career with the city of Los Angeles include completion of the Tillman Phase II Primary Tank Expansion; getting sodium bisulfite approved for use in dechlorination at the LA Glendale Treatment Plant, completion of the East Central Interceptor Sewer Project (ECIS); getting North Outfall Sewer (NOS) Maze III designed and bid; approval of products for non-circular pipe slip-lining; approval of use of precast inserts for live sewer connections; coordinating the BOE Wastewater Training Program; approval of MCA requirement for Metro to encase sewers with rail lines crossing over them; and standardization of material properties for cured-in-place pipe (CIPP) liner design.
He says the assay could eliminate the need for bisulfite conversion of DNA, fluorescent labeling, and polymerase chain reaction (PCR).
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