blot analysis


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blot analysis

n
(Biochemistry) biochem a technique for analysing biological molecules, such as proteins (Western blot analysis), DNA (Southern blot analysis), and RNA (Northern blot analysis), involving their separation by gel electrophoresis, transfer to a nitrocellulose sheet, and subsequent analysis by autoradiography. Also called: blotting
References in periodicals archive ?
The aim is to replace the currently used multi-step Western blot analysis with the appropriate instrumental method, which does not require manipulation of gels, the use of different device sequences and manually demanding technological procedures.
The homogenate was centrifuged at 12,000 g for 20 min at 4 C and the resulting supernatant was used for dot blot analysis.
The reaction mixture was centrifuged at 12,000 rpm and 4[degrees]C for 5 min, and the supernatant was used in western blot analysis.
Results from the western blot analysis revealed that both mAbs reacted with the 32kDa and 37kDa protein in raw horse and poultry meat, respectively.
As shown in Figures 1(c) and 1(d), Western blot analysis indicated that the TGF-[beta] treatment significantly downregulated the KLF-4 protein level in LA-4 cells, dose-dependently (p < 0.
Western blot analysis was performed using the following antibodies: transforming growth factor-[beta] (TGF-[beta], R&D Systems, MN, USA), collagen IV (Abcam, Cambridge, UK), fibronectin (Proteintech Group Inc.
Expression of RhoA in the cytoplasm and membrane as well as ROCK II was also determined by Western blot analysis.
Methods: We examined the effect of the OJE on copper, zinc-superoxide dismutase (SOD1), manganese superoxide dismutase (SOD2), catalase (CAT), and glutathione peroxidase (GPx) in the rat liver using immunohistochemistry and western blot analysis.
Further PCR Southern blot analysis displayed that the gene aiiA segments were transformed into T0 plants.
Western blot analysis confirmed that the expression of anti-apoptotic proteins decreased and the pro-apoptotic proteins increased in the presence of UV radiation.
Western blot analysis suggests that cadmium induces cathepsin b expression, indicating induced autophagy in osteoblasts.