The common indicator solutions include thymol blue, methyl orange, bromthymol blue, phenolphthalein, and bromphenol blue
. These solutions are basically used in analytical chemistry when titrations are needed, during which a color change can indicate the alkalinity or acidity.
Four microliters of PCR products containing heteroduplexes were mixed with 3 ml of stock loading buffer (stock solution of 30% glycerol, 0.25% bromphenol blue
, 0.25% xylene cyanol).
Five microliters of the reaction product was mixed with 1 ul of loading buffer (60% glycerol, 0.25% bromphenol blue
, 0.25% xylene cyanol), and then the mixture was run on a 1,5% horizontal agarose gel (containing ethidium bromide at 1 ug/ml) at 100 V for 50 min in 50 mM Tris-borate-EDTA buffer (pH 8.0).
Each protein sample was dissolved in sample buffer containing 0.2 M Tris buffer (pH 6.8), 10% (w/v) SDS, 20% (w/v) glycerol, 0.05% (w/v) bromphenol blue
at the ratio of 5:1 (v/v).
Prior to electrophoresis, 30 [micron]g soluble protein extract was diluted with sample loading buffer (0.06 M Tris-CI, pH 6.8, 5% [beta]-mercaptoethanol, 10% glycerol, 2% SDS, 0.01% bromphenol blue
) and heated at 100[grados]C for 5 min.
The cytochemical staining and measurement of protein with mercuric bromphenol blue
Items there included a limited edition Andy Warhol Dollar print worth PS13,500, a Bromphenol Blue
dyed print by Brit art star Damien Hirst valued at PS6,000, and another piece from street artist Banksy.
Protein collection was accomplished by using dialysis tubing, with samples marked by bromphenol blue
Antigens were diluted in phosphate-buffered saline (PBS; the formulation of PBS, which was obtained as tablets from Sigma, is 0.01 mmol/L phosphate buffer, 0.0027 mmol/L KCl, 0.137 mmol/L NaCl, pH 7.4; the formulation and source are the same when PBS is mentioned elsewhere in this article) with the exception of dsDNA, which was diluted with 3x sodium chloride/sodium citrate buffer (i.e., 0.45 mol/L sodium chloride, 0.045 mol/L trisodium citrate, pH 7); all antigen dilutions contained 25 mL/L glycerol and 50 mg/L bromphenol blue
. Antigens were deposited as 12-nL nanodots via noncontact printing with a BioDot AD3200 equipped with a microsolenoid ink jet and a 100-[micro]m ceramic tip.
PTMs did not stain with bromphenol blue
for protein, and a Bradford assay indicated that only about 3.5% of total dry weight was soluble protein.