Determination of cell concentrations using haemocytometer according to Fuchs Rosenthal and Burker
Standard procedures were employed for total and differential cell numbers evaluation: for valuation of leukocytes, sediment cells from BALF were suspended in PBS treated with Turk reagents and calculated in optical microscopy in a Burker
EJ, Blumenthal JA, Feldman M, Thyrum E, Mahanna E, White W, et al.
The enumeration was performed by using Burker
chamber and sperm count was calculated using weight of testis and dilution volumes.
KBr-IR and [sup.13]C, [sup.1]H NMR spectra were obtained by JASCO FT-IR-4100 spectrophotometer (400-4000 [cm.sup.-1]) and Burker
500 MHz, respectively.
The protocol was designed to validate an automated method for MSC counting by "NucleoCounter[R]" system (ChemoMetec, Allerod, Denmark) in terms of accuracy, precision, and linearity in comparison to the manual cell count method by the hemocytometer (Burker
chamber was used to determine the number of cells per 1 mL of solution.
The DNA content was expressed as [micro]g DNA/[10.sup.6] cells counted, in each well, using a Burker
chamber and trypan blue.
1H-NMR spectra were recorded in CDCl3 on Burker
spectrometer, operating at 400 MHz at 25 AdegC.
Cell concentrations, in number per milliliter, were determined by use of a hemocytometer (Burker
) by counting 150-300 cells as described elsewhere (29).