The gizzard, breast meat, bursa of Fabricius
, liver, and abdominal fat were then removed by trained personnel and weighed and analyzed immediately.
For instance, the bursa of Fabricius
is present in hens but not in mammals.
It targets the bursa of fabricius
which is an organ found in young birds developing immune system.
The tissues of trachea, spleen, brain, lung, liver, bursa of Fabricius
, proventriculus, and caecal tonsils were collected and fixed in 10% buffered formalin.
We collected tissue samples (lungs, trachea, heart, brain, liver, kidneys, spleen, crop, proventriculus, gizzard, small and large intestine, pancreas, thymus, bursa of Fabricius
, adrenal glands, and gonads) from each bird, fixed the samples in neutral-buffered 10% formalin solution, and processed them by standard methods for histopathologic examination.
Infectious bursal disease (IBD) is an acute, highly contagious infection of young chickens (Gallus gallus domesticus), caused by infectious bursal disease Virus (IBDV), which primarily targets the lymphoid tissues of bursa of Fabricius
(Kaufer and Weiss, 1980).
Compared with mammals, avains have a specific central humoral immune organ which is named the bursa of Fabricius
(BF) and plays an important role in immune resistance [1,2].
These results agreed with previous studies that showed aflatoxin (8ppb) lead to severe depletion of lymphoid tissue such as bursa of fabricius
and spleen  and aflatoxin (50 [micro]/kg) revealed vacuolar degeneration and heterophils infiltration in other organ (liver) , while, contaminated feed contain 3 mg/Kg aflatoxin lead to significant depletion in the bursa of fabricius
and severe damage in the liver .
To evaluate the tissue expression profiles of IFITM1 and IFITM3, various tissues, including the brain, bursa of Fabricius
, cecum, cecal tonsil, gizzard, heart, Harderian gland, kidney, liver, lung, muscle, pancreas, proventriculus, small intestine, skin, spleen, thymus, and trachea, were collected from two-week-old gosling and adult goose.
Histopathologic examination revealed that the most important changes were located in the crop, bursa of Fabricius
, and lungs with presence of amorphic basic intracytoplasmic inclusions.
Bursa of Fabricius
(BFs) samples collected from birds and fresh carcasses from infectious bursal disease (IBD) outbreaks was used as sources of respective virus samples and those from the same farm were pooled and homogenized together.
Since the primary site of infection and inducement of lesion by IBDV is bursa of fabricius
, the effects on the immune system may be significantly suppressive.