One of the [Ca.sup.2+] regulation mechanisms is binding of calcium to calmodulin
Gonadotrophin-releasing hormone signalling downstream of calmodulin
Another two SNPs were located on the positions of 1.39 Mb and 2.71 Mb of chromosome 8 respectively, with the first SNP locating on the 14th intron of calmodulin
regulated spectrin associated protein family member 2 (CAMSAP2) and the other locating on the 4th intron of potassium sodium-activated channel subfamily T member 2 (KCNT2).
is a structurally conserved and functionally preserved protein that is encoded by the CALM3 gene.
Investigating the disorder-order transition of calmodulin
binding domain upon binding calmodulin
using molecular dynamics simulation.
For individual 1083, calmodulin
3 (CALM3) and phospholamban (PLN) were differentially expressed following treatment with isoproterenol.
The PCR reaction amplified the regions ITS1-5,8S-ITS2 of ribosomal DNA, partial P-tubulin gene, and partial calmodulin
gene with the following pairs of primers: ITS1 5'TCCGTAGGTGAACCTGCGG3' and ITS4 5'TCCTCCGCTTATTGATATGC3' (White et al., 1990), Bt2a 5'GGTAACCAAATCGGTGCTGCTTTC3' and Bt2b 5ACCCTCAGTGTAGTGACCCTTGGC3' (Glass and Donaldson, 1995), and CL1 5'GARTWCAAGGAGGCCTTCTC3', and CL2A 5'TTTTGCATCATGAGTTGGAC3' (Mule et al., 2004), respectively.
These higher-molecular-weight bands might represent fusion proteins comprising neurogranin, calmodulin
, and other calmodulin-binding proteins (30).
Rapid recovery is Ca and calmodulin
dependent, (26) and it has been shown that Munc13-1, which has a calmodulin
binding site, is responsible for rapid recovery from depletion (43) (see also Fig.
Expression and Purification of CaMKII and Calmodulin
. Recombinant human CaMKII[delta] (NCBI RefSeq: NP_742113.1) with an N-terminal 6xHN tag was integrated into a baculoviral construct (BacPAK9-6xHN), amplified in Sf9 insect cells (expression systems), and expressed in Hi5 (T.
Anderson, "The role of calmodulin
kinase II in myocardial physiology and disease," Physiology, vol.