Data analysis: FASTQ files were separated by CASAVA
and trimmomatic tool was used to filter the low quality reads (Q>30).
1.8 analysis package (Illumina[R], San Diego, CA, US) was used to demultiplex the sequencing reads.
(version 1.8.2, Illumina) was used to convert .bcl (base call) files generated from the MiSeq instrument into FASTQ files containing short-read data and to perform sample de-multiplexing.
Ate ao casamento, o kyrios era o pai da jovem; quando esta se casava
, a funcao passaria a caber ao marido.
Basecalls were performed using CASAVA
The Illumina Sequencing Control Software (SCS) v2.8, the Illumina Off-Line Basecaller Software (OLB) v1.8, and the Illumina Consensus Assessment of Sequence And VAriation (CASAVA
) v1.8 were used to produce 100 bp sequence reads.
(bis) Se eu fosse um moco sortero, eu casava
com essa moca Eu so serrador do mato grosso, aiaaaaaaaaiiiiiiii Respossta E de vera meu patrao, aaaaaaaiiiiiiii Assunta o que eu vo fala, aaaaaaaiiiiiiii Dandao, eu so serrador do mato grosso, aiaaaaaaaaiiiiiiii Moca bunita, Rusarios branco, com uma medaia no pescoco (bis) Se eu fosse um moco sortero, eu casava
com essa mooooca, aiaaaaaaaaiiiiiiii Dandao, eu so serrador do mato grosso, aiaaaaaaaaiiiiiiii (Cancao "Serrador do mato grosso", Marombeiros dos Quilombos do Alagadico, Municipio de Minas Novas, Vale do Jequitinhonha/MG, DINIZ, 2013b).
After completion, sequence reads were filtered for read quality, base called, and demultiplexed by using Casava
version 1.8.2 software (Illumina).
Food groups Proportion of respondents Milk Whole milk 4.5 Skinned milk 65 Both 12.3 None 6.8 Do not know 11.4 Yoghurt Low fat and sweetened 12.3 Low fat and unsweetened 60.9 Both 16.8 None 5.5 Do not know 4.5 Breakfast Oats porridge 10.9 Rice porridge 21.4 Both 62.3 None 0.9 Do not know 4.5 Roots tubers Potatoes 48.6 Casava
1.8 Both 31.4 None 11.8 Do not know 6.4 Note: Table made from bar graph.
Raw data were demultiplexed and converted to FASTQ.gz format using CASAVA