For cholesterol, the assay consisted of an enzymatic, calorimetric method whereby cholesterol esters were cleaved by the action of cholesterol esterase
to yield free fatty acids and cholesterol.
These methods use combinations of surfactants, proteins, and ionic components to selectively discriminate between HDL-C lipoproteins and other lipoproteins in their reaction with cholesterol esterase
1982, "Hydrophobic ionic chromatography: its application to microbial glucose oxidase, hyaluronidase, cholesterol oxidase and cholesterol esterase
," Journal of Biochemistery.
For estimation, extraction of each sample was done with one disc only and 50 [micro]l of the extract was added to 1 ml of the commercially available enzymatic reagent kit containing cholesterol esterase
, cholesterol oxidase, peroxidase, phenol and 4-amino antipyrine in PIPES buffer (Lot no.
Enzyme induced biodegradation of polycarbonate-polyurethanes :dose dependence effect of cholesterol esterase
We determined the amount of free and esterified cholesterol (total cholesterol) using a colorimetric method (Roche) in the presence of cholesterol oxidase and cholesterol esterase
and then measured the absorbance at 405 nm.
One of those enzymes, cholesterol esterase
, softens the polymers, or resins, used in dental work, Shiu Heman Tsang of the University of Toronto in Ontario reported last week at the American Association for Dental Research in San Antonio.
Reagent 1 consisted of 600 U/L cholesterol esterase
, 500 U/L cholesterol oxidase, 1200 KU/L catalase, 2 mmol/L N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline, sodium salt, and dihydrate in PIPES buffer (50 mmol/L, pH 7.
The main objective of this work was to prepare an enzymatic membrane where, cholesterol oxidase and cholesterol esterase
were immobilized for the determination of cholesterol in serum.
On the other hand, BuChE may play a role in lipid metabolism (21), whether directly (19) or through a synergistic action with cholesterol esterase
, as has been demonstrated in saliva (22).
This method uses a detergent polymer mixture (reagent 1) to release cholesterol from non-LDL lipoproteins that reacts with cholesterol esterase
, cholesterol oxidase, peroxidase, and 4-aminoantipyrene to produce colorless products.
This assay is derived from the specific detection of cholesterol in HDL particles, achieved by polyethylene glycol-modified cholesterol esterase
and cholesterol oxidase and masking of other lipoprotein particles with sulfated cyclodextrin.