mLDLc and HDLc were measured by cholesterol esterase
method on ADVIA 1800 clinical chemistry system.
Total cholesterol assays underwent Abell/Kendall standardization and an enzymatic, colorimetric method (cholesterol esterase
, peroxidase [CHOL2]).
Total cholesterol and TG were measured by Cholesterol Oxidase (CHOD)-PAP and glycerol-3-Phosphate Oxidase (GPO)-PAP method on Selectra-ProM; while LDL-c and HDL-c were analysed by cholesterol esterase
method on ADVIA 1800 Chemistry System respectively.
ensures that cholesterol oxidase reacts with all cholesterol.
oxidase-peroxidase-amidopyrine method was used to assess serum cholesterol, and for measurement of serum TG, glycerol phosphate oxidase-peroxidase-amidopyrine method was used.
Further, the studies conducted worldwide so far on antilipidemic activity of authenticated Ceylon cinnamon did not address its effect on HMG-CoA reductase, cholesterol esterase
, and cholesterol micellization inhibitory activities and bile acid binding.
Total cholesterol was determined using the cholesterol esterase
method on a semi-automated analyser.
The method principle is a homogenous enzymatic cholesterol assay (cholesterol esterase
, cholesterol oxidase/peroxidase coupling reaction) modified by the addition of a non-ionic detergent, a sugar compound and magnesium which enables the selective determination of LDL (6).
These methods use combinations of surfactants, proteins, and ionic components to selectively discriminate between HDL-C lipoproteins and other lipoproteins in their reaction with cholesterol esterase
and Horio, T., 1982, "Hydrophobic ionic chromatography: its application to microbial glucose oxidase, hyaluronidase, cholesterol oxidase and cholesterol esterase
," Journal of Biochemistery., 91, pp.
For estimation, extraction of each sample was done with one disc only and 50 [micro]l of the extract was added to 1 ml of the commercially available enzymatic reagent kit containing cholesterol esterase
, cholesterol oxidase, peroxidase, phenol and 4-amino antipyrine in PIPES buffer (Lot no.
The influence of protein adsorption and surface modifying macromolecules on the hydrolytic degradation of a poly(ether-urethane) by cholesterol esterase
. Biomaterials 2003;24:121-130.