The PCR product was purified using Quiagen kit as per the manufacture instruction and sent to Chromous
Bonner, The Exchange Reaction Between Chromous
and Chromic Ions in Perchloric Acid Solution, J.
The authors wish to sincerely thank Chromous
Biotech Ltd, Bangalore, India, for sequencing the amplicons.
For the molecular identification of the isolate with piperine production, genomic DNA was purified using the Chromous
Biotech Fungal gDNA Mini spin kit (Category number RKT41).
Twenty [micro]L reaction mix contained 1X PCR buffer, 0.75 units of Taq DNA polymerase (Sigma Aldrich, USA), 200 [micro]M dNTPs (Sigma Aldrich, USA), 0.5 [micro]M each primer (Chromous
Biotech, Bangalore, India), and 50ng/[micro]L of genomic DNA.
Transfer of two electrons from two monomer ions radical by H2CrO4 produces para semidine salt along with chromous
acid H2Cr2O3 (Cr(IV)).
The method was performed at one of the commercial laboratories, namely, Chromous
The successful PCR amplicons (40 [micro]L post-PCR) were sent for direct sequencing to the Chromous
The PCR products were purified using gel extraction kit (Chromous
For this fungal, genomic DNA was isolated by using Fungal gDNA extraction kit (Chromous
Pyrite removal from kerogen without altering organic matter: the chromous
chloride method // Energ.
The amplification product was purified by Chromous
PCR clean up Kit (#PCR 10) according to manufacturer's instructions.