and tryptic hydrolysis: The protein (10 mg) was solubilized in 1mL of 0.1M sodium phosphate buffer, pH 8.0.
Magdolen et al., "Chymotryptic
specificity determinants in the 1.0 A structure of the zinc-inhibited human tissue kallikrein 7," Proceedings of the National Academy of Sciences of the United States of America, vol.
Digestion of the Trypsin-resistant "Core" of Haemoglobins A and S and.
It is formulated with a high-purity recombinant trypsin, free of chymotryptic
activity, to ensure high fidelity digestion.
Molecular cloning of acDNA that encodes a serine protease with chymotryptic
and collagenolytic activities in the hepatopancreas of the shrimp Penaeus vannameii (Crustacea, Decapoda).
After LLVY chymotryptic
cleavage by the 20S proteasome, AMC is released and emits fluorescence that can be read in a fluorometer by using a 380/460 nm excitation and emission filters, in this case in a Tecan Infinite F200 PRO microplate reader (Tecan Italia srl, Cernusco sul Naviglio, Italy).
Kang, "Covalent structure of collagen: amino-acid sequence of chymotryptic
peptides from the carboxyl-terminal region of alpha2-CB3 of chick-skin collagen," European Journal of Biochemistry, vol.
FLG has several site-specific functions governed by the program of epidermal terminal differentiation in which a number of proteases are involved, such as corneum trypsin- and chymotrypsin-like enzymes (stratum corneum tryptic enzyme/kallikrein 5 [KLK5] and stratum corneum chymotryptic
enzyme/KLK7),[sup],, channel-activating serine protease1 (CAP1),[sup] matriptase (coded by ST14),[sup] and cysteine protease caspase-14[sup] and their key protease inhibitor lymphoepithelial Kazal-type-related inhibitor (LEKTI) (encoded by serine protease inhibitor Kazal-type 5 gene/ SPINK5 ).[sup],, All of the proteases can enhance FLG degradation processing.
digestion, myosin can be divided into heads and a tail.
Effect of unpalatable diets, food restriction and saccharin-adulterated diet on tryptic, chymotryptic
, and amylolytic activity in pancreas, intestine and feces of rats.
These results suggest that over 75% of the peptides identified in ErbB2 samples digested by trypsin or by Asp-N and 58% of chymotryptic
peptides can quantify a twofold change between two conditions (Figure 4(b)).
Murphy et al., "Genetic association between an AACC insertion in the 3/UTR of the stratum corneum chymotryptic
enzyme gene and atopic dermatitis," Journal of Investigative Dermatology, vol.