restriction site

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Noun1.restriction site - the specific sites at which a restriction enzyme will cleave DNA
site, situation - physical position in relation to the surroundings; "the sites are determined by highly specific sequences of nucleotides"
Translations
site de restriction
References in periodicals archive ?
Influenza virus HA requires activation by host proteases at a conserved cleavage site between the HA1 and HA2 subunits.
BbvI has lost its cleavage site either in one allele or both alleles.
Modification of Sema3C at the furin cleavage site [sub.742][RNRR.sub.745] was confirmed by sequencing (Figure 1A).
The ELM showed CLV_NDR_NDR_1 motifs matched sequence with amino acid residues RRT (878-880), a functional motif of N-Arg dibasic convertase (nardilysine) cleavage site (Xaa-I-Arg-Lys or Arg-I-Arg-Xaa) in the extracellular Golgi apparatus and cell surface.
While we may have had to move our cleavage site a short distance away from where we want the exact change, the HDR will allow us to engineer back down across the exact nucleotide(s) of interest.
Table II.- Highest nucleotide homology and cleavage site motifs of Pakistani IBV compared to sequences available in GenBank.
Interestingly, the major determinant of NDV virulence has been traced to be the amino acid composition of its fusion protein cleavage site [25].
The arrowhead indicates the expected Cas9 cleavage site. The protospacer adjacent motif (PAM, red underline) is the motif required for Cas9 nuclease activity.
In addition, the cleavage site in cTnT was localized by use of mass spectrometry and probing of different proteolytic fragments with the use of various antibodies.
The cleavage site in PPO has been experimentally confirmed in several insect species, including [sup.51][Arg-Phe.sup.52] in B.
Determination of the second autoproteolytic cleavage site of cephalosporin C acylase and the effect of deleting its flanking residues in the a-C-terminal region, J.
The signal peptide cleavage site was predicted using SignalP server (version 4.0), which is based on neural network method 24.