The inoculated birds were then closely observed for development of any clinical signs, lesions, mortality, daily cloacal
temperature, daily food and water intake.
To evaluate attenuation of the manual restraint-induced stress response, heart rate, respiratory rate, and cloacal
temperature were measured over a 15-minute period.
temperature (CT) was measured using a digital clinical thermometer that was inserted to a depth of three centimeters for 2 min.
At the third sampling stage, for each poultry type, we randomly selected 5 birds from each of the stalls selected for that type and collected cloacal
and oropharyngeal swab specimens from each of the selected birds.
Samples of ingesta from necropsied birds, cloacal
swabs from live birds were collected and stained by gram staining as per standard protocol.
These reptiles can absorb oxygen through the skin on their neck and cloacal
areas, allowing them to stay submerged under water for prolonged periods of time and hibernate.
Se realizo lavado del area cloacal
con abundante agua y se desinfecto con alcohol, se tomo la muestra de materia fecal por medio de un hisopado cloacal
(Figura 2A y 2B), posteriormente el hisopo se introdujo en un medio de transporte (500 [micron]L de Caryblair[R]), y fue refrigerado a 4[grados]C hasta el momento del procesamiento.
Oronasal (ON) swabs taken from the choanal slit and cloacal
(CL) swabs using Copan FLOQSwabs[TM] (ref 552C, Copan Diagnostics Inc., CA, USA) were collected daily between day 1 and 7 and then at day 10 pch.
 The rectum and anus are believed to develop from the dorsal portion of the hindgut or cloacal
cavity when lateral ingrowth of the mesenchyme forms the urorectal septum in the midline.
Lourdes Socorro, Dumaguete City veterinarian, on Thursday said blood samples and cloacal
swabs taken from the birds would be submitted to the local office of the agriculture department.