After incubation, a presumptive count of colony-forming units (CFU) was performed, the presumptive colonies were selected and transferred to Brain Heart Infusion broth (Difco, Sparks, USA), and confirmation was performed by phenotypic tests of Gram staining, catalase and coagulase
The microorganisms that were isolated as single or multiple growths in order of frequency were Coagulase
Negative Staphylococcus spp.
cepacia: Burkholderia cepacia, ICU: Intensive care unit, CTVS: Cardiothoracic and vascular surgery Table 3: List of organisms isolated from different environmental samples Type of environmental sample Organisms isolated Lid (inner and outer surfaces) of Coagulase
negative Staphylococci, blood culture bottles Enterococcus spp.
Methicillin-resistant Staphylococcus pseudintermedius (MRSP) is coagulase
positive staphylococci identified by Devriese et al.
During observations, 39 specimens were growth positive; the biochemistry of isolates revealed that they were coagulase
, catalase and oxidase negative, TSI, gel hydrolysis positive and were able to ferment glucose, lactose, maltose, galactose, fructose, sucrose, starch and raffinose.
lugdunensis produces a bound coagulase
but no free coagulase
, and thus the short coagulase
test may be positive but the long coagulase
test--which definitively identifies a species as coagulase
-positive--will remain negative.
The scarcity of reported cases of human infection, combined with variable biochemical properties of this germ in routine identification tests, mainly concerning coagulase
activity, may favor misdiagnosis.
The micro-organisms most commonly isolated from otitis in dogs have been coagulase
positive staphylococcal infections are an important cause of death.
negative staphylococci as causative agents of urinary tract infections- prevalence and resistance status in IGIMS, Patna.
In a comparison of virulence factors between biofilm-forming isolates and planktonic cells, significant difference was seen for plasma coagulase
and hemolysin production.
Staphylococcal isolates were subjected to coagulase
test to differentiate Staphylococcus aureus from other Staphylococcal species.