The procedures included induction of callus (A), subculture (B), cocultivation
and Basta screening (C), differentiation (D), rooting (E), and planet acclimatization (F).
We evaluated the biological characteristics of this model and the effects on the encapsulated cells upon cocultivation
One such strategy for beta cells is cocultivation
or cotransplantation with human mesenchymal stem/stromal cells (hMSCs), which play a key role in regenerative medicine and tissue engineering.
After 24 h cocultivation
at 37[degrees]C, CCK8 assay was used to measure the cell viability.
In addition to the IPEC-J2/MoDC cocultures, monocultures of IPEC-J2 cells or MoDC were also included as controls to assess the influence of cocultivation
on the reactivity of each cell type (Figures 1(a) and 1(b)).
In this work, we delve deeper into the study of these clones, analysing their effect on purified populations of T lymphocytes, the cytokine environment resulting from cocultivation
with PBMC, the ability of clones to modify the Treg population, the effect of CM on PBMC and Treg proliferation, and finally, the effect of these clones on the viability of PBMC exposed to proapoptotic stimuli.
Ozuolmez and colleagues observed a similar shift from high numbers of sulfate-reducers to higher numbers of methanogens during a cocultivation
of Methanosaeta concilii and Desulfovibrio vulgaris on acetate .
tumefaciens strain, infection time, duration of cocultivation
, and the selection medium have important bearing on transformation efficiency.
They showed that cocultivation
of STZ-injured islets and MSCs increased expression of IL-6 and TGF-[beta]1 in the culture medium besides the expression of antiapoptotic genes.
conditions selection system and cultivars are some of the important factors which considerably affect the Agrobacterium-mediated transformation efficiency (Joyce et al.