To challenge our coculture
model, we additionally included (4) prohaptens (3-aminophenol, resorcinol and acetaminophen) that remained negative with regard to the induction of keratinocyte responses even after exposure in the presence of additional S9 mix (modified KeratinoSens (Natsch and Haupt, 2013)).
Cell flasks were incubated 5 hours prior to flow cytometry analysis and cells in plates were incubated 24 hours for coculture
Therefore, to mimic in vivo conditions, strains were inoculated individually or together (coculture
) at [10.sup.6] colony forming units (CFU) for PAO1 and [10.sup.2] CFU for VPI 4355.
Furthermore, in the in vivo environment, these cells do not contact directly, so their coculture
may influence and distort the results.
Previous research replaced the transwell PET membrane with an electrospun gelatin scaffold, styled "biopaper," in an endothelial/astrocyte coculture
in vitro model .
demonstrates that treatment with antioxidants not only increases proliferation and mitochondrial integrity of human mesenchymal stem cells (hMSCs) but also enhances the therapeutic potential of hMSCs by promoting the formation of tunneling nanotubes (TNTs) between hMSCs and oxidatively injured cells in a coculture
In this study, we examined the key factors in this system, namely, OPG and RANKL, in an in vitro coculture
model using the MDA-MB-231 breast cancer and the mouse osteoblast MC3T3-E1 cell lines.
with mitotically inactivated MEFs or PGCs, blastocysts were seen attached and from it outgrowths were observed; then it was kept in culture for 10 days till it reached approximately 1 cm diameter.
Schmal, "The trans-well coculture
of human synovial mesenchymal stem cells with chondrocytes leads to self-organization, chondrogenic differentiation, and secretion of TGF[beta]," Stem Cell Research & Therapy, vol.
in potting soil samples (14-16), and the use of amebal coculture
techniques has shown promising results in recovering L.
system comprising human intestinal epitheliallike Caco-2 cells and phorbol myristate acetate (PMA)-stimulated THP-1 macrophage cells was used.
Final density of splenocytes was adjusted to 2.5 x [10.sup.5] cells per well for coculture