Appendix O: Ohio water microbiology laboratory coliphage
detection by USEPA method 1602: Single-agar layer (SAL) procedure.
Kohn, "Inactivation of MS2 coliphage
in Funton and Fenton-like systems: role of transition metals, hydrogen peroxide and sunlight," Environmental Science & Technology, vol.
We are aware of only two peer reviewed studies that estimated health risks posed by exposure to zoonotic pathogens in land-applied dairy manure, including a study that generated risk estimates for center pivot irrigation of dairy manure based on an air dispersion model (Dungan 2014) and one that estimated risks based on an empirical fate and transport model for coliphage
spiked into groundwater that was sprayed using conventional tanker equipment (Brooks et al.
Hatch, "Survival of T3 coliphage
in varied extracellular environments," Journal of Applied Microbiology, vol.
Pratt, "Some physicalchemical and biological properties of the rod-shaped coliphage
M13," Virology, vol.
coli (CFU/100 mL) Membrane Filter 9222B Coliphage
(UFP/100m CETESB, 1990--L5.225 [L.sup.-1]) Clostridium perfringens Multiple tubes (CETESB, (MPN/100m[L.sup.-1]) 1993--L5.213) Table 2.
Dubois E, Hennechart C, Deboosere N, Merle G, Legeay O, Burger C, Le Calve M, Lombard B, Ferre V, Traere, O (2006) Intra-laboratory validation of a concentration method adapted for the enumeration of infectious F-specific RNA coliphage
, and hepatitis A virus from inoculated leaves of salad vegetables.
coli, enterococci, male-specific coliphage
) were analyzed for spatial and temporal trends.
Evaluation of four different systems for extraction of RNA from stool suspensions using MS-2 coliphage
as an exogenous control for RT-PCR inhibition.
Functional and genetic analysis of regulatory regions of coliphage
H-19B: location of Shiga-like toxin and lysis genes suggest a role for phage functions in toxin release.
Bovre and Szybalski  found that the central b2 region in the coliphage
A genome could produce two opposite oriented mRNAs, one originating on the plus strand and the other on the minus strand, that partially overlap with each other.
However, the results of the study did indicate a possible pattern between gastrointestinal illness and both [F.sup.-] coliphage
and Giardia spp., both being detected on 4 of the 15 total study days (Table 3) , and both were detected on 3 of the 5 days characterized by the highest excess gastrointestinal illness (see the number represented by asterisk (*) in Table 3).