The Vivo nanofat was prepared by digesting the rinsed fat tissue with 1 ml of 0.2 mg/ml collagenase
I (Sigma, Germany) resuspended to a final volume of 20 ml and incubated at 37[degrees]C for 15 min (we designed a short-time digestion process to save the viability of the adipocytes).
The degradation assay lasted for 4 hours and every 30 minutes measurements were conducted to check the resistance of the gels to degradation induced by the collagenase
The minced tissue was enzymatically digested by a mixture of trypsin, DNase I, and collagenase
IV at 37[degrees]C for 30 min under shaking conditions.
McCulloch, "Evidence of a direct relationship between neutrophil collagenase
activity and periodontal tissue destruction in vivo: role of active enzyme in human periodontitis," Journal of Periodontal Research, vol.
The residual tissue was washed with PBS, digested with collagenase
again at 4 AdegC overnight, and then processed as described above.
Xiaflex is purified collagenase
clostridium histolyticum, isolated and purified from the fermentation of Clostridium histolyticum bacteria (Endo Pharmaceuticals, Inc., 2017).
Studies on neutrophil collagenase
and MMP-18 in clear cell renal cell carcinoma did not demonstrate any increase in their activity in primary tumour tissue or metastases as compared to normal tissue [3,32].
The elevation of cortisol inhibits fibroblast function and increases matrix metalloproteinases (collagenase
MMP-1, MMP-13, and, in particular, collagenase
2 (MMP-8) play a special role in inflammatory and destructive processes in periodontium since the substrates for these enzymes are collagen types I, II, III, and IV which are important proteins of periodontal attachment apparatus and the soft tissues around implants [19-21].
Bacterial endotoxin (Sigma, L2630, 10 mg), type I collagenase
(Sigma, lot number: C0130), Masson staining kit (Beijing Reagan, lot number: DC0032), rabbit TNF-[alpha] kit (R&D Systems subpackage, rb201510200842), IL-1[beta] kit (R&D Systems subpackage, rb201510190821), and IL-4 kit (R&D Systems subpackage, rb201510270951) were the major agents used in this study.
The overall objective of this study was to evaluate the effect of enzyme exposure, incubation time, and additional physical agitation cycles in optimizing chondrocyte isolation from nasal cartilage biopsies using the commonly employed collagenase
cilicica on hyaluronidase, collagenase
and elastase enzymes, which are the major enzymes responsible for dehydration of the skin, were investigated.