column chromatography


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Related to column chromatography: Thin layer chromatography
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Noun1.column chromatography - chromatography that uses selective adsorption by a column of powders
chromatography - a process used for separating mixtures by virtue of differences in absorbency
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References in periodicals archive ?
Summary: Silica gel column chromatography of the ethyl acetate fraction of methanol extract of Vincetoxicum stocksii resulted in the separation of three new rarely occurring natural products; [4-(4-(methoxycarbonyl)benzyl)phenyl] carbamic acid (1), bis[di-p-phenylmethane]ethyl carbamate (2), methyl 2-hydroxy-3-(2-hydroxy-5-(3-methylbut-2-enyl)phenyl)-2-(4-hydroxyphenyl) propanoate, stocksiloate(3), along with five known compounds; 1-(4-hydroxy-3-methoxyphenyl)-1,2,3,-propanetriol (4), feruloyl-6-O-[beta]-D-glucopyranoside (5), 4-hydroxy-3,5-dimethoxybenzoic acid (6), apocynin (7) and vincetomine (8).
Column chromatography is a common and useful separation technique in organic chemistry and can be used in both large and small scale.
The dissolved cerebroside which was obtained by using chloroform and methanol (2:1/v:v) was subjected to column chromatography on silica gel, and then was eluted with chloroform-methanol-H2O (20:1:0.2), by which 3 fractions (1,2,3) was afforded.
Zhu [18] separated shale oils from mountainous area (land) and coastal shelf (sea) oil shale rocks into four fractions by using silica gel column chromatography. The components of each fraction were identified by gas chromatography-mass spectrometry (GC-MS).
Extraction of the antimicrobial compound from culture supernatant using different solvents and purification by column chromatography
Column chromatography was performed using silica gel 60 (Merck, 70-230 mesh).
On the basis of the previous studies, our research team further used the extraction technique to roughly extract Dahuang first; then isolated and purified it by repeated silica gel column chromatography, sephadex column chromatography, ODS C18 reversed phase silica gel column chromatography and MCI column chromatography; and finally identified it by [sup]1 H-NMR,[sup]13 C-NMR, and DEPT nuclear magnetic resonance spectroscopy.
Toluene, ethyl formate, chloroform (CH[Cl.sub.3]), ethyl acetate (EtOAc), methanol (MeOH), ethanol (EtOH), distilled water, and formic acid were used as the open column chromatography and thin layer chromatography.
The presentation includes a brief historical perspective and schematic illustration of column chromatography. The design and operation of modern chromatographic instrumentation is described, including individual components.
To use the ideal column in the separation of Malathion is recommended to work with high values of temperature to avoid dispersion of the compound through the entire column chromatography, and thereby obtain an extraction point.
Column Chromatography separation was done involving the same principles as Thin Layer Chromatography (TLC), but can be applied to separate larger quantities than TLC.