At least two of those fires were lit in Henderson's complexone
of them in an adjacent building and the other in her buildingher estate alleged.
The routine methods for measuring total calcium include azo arsenic III colorimetry, O-cresolphthalein complexone
(OCPC) colorimetry, and the methylthymol blue colorimetric method.
A 10-mL urine subsample and serum samples stored at -20[degrees]C were analyzed for [Na.sup.+] (ion-selective electrode), [K.sup.+] (ion-selective electrode), [Cl.sup.-] (ion-selective electrode), [Ca.sup.2+] (o-cresolphthalein complexone
), P[O.sup.3-.sub.4] (molybdate reaction), and [Mg.sup.2+] (xylidyl blue reaction) concentrations with an electrolyte analyzer (AC9000, Audicom medical technology co., LTD, Shanghai, China).
* Measurement of calcium in serum was done using Ortho Cresolphthale in complexone
method (OCPC method).
and Alkaline Phosphatase Activity Assay.
The estimation of PAP was done by Kinetic method , calcium by O-cresolphthalein complexone
method and End Point Assay , and the level of phosphorus was done by UV-End point method .
All miniature pigs were injected intravenously (10ml/min) with three different fluorochromes xylenol orange (6%, 2-5g/animal), calcein green (1%, 0.8-1.5 g/ animal), and alizarine complexone
(3%, 1-1.5 g/animal) 14, 28, and 84 days after implantation.
Inorganic phosphorus was measured by the direct molybdate method (Inorganic phosphorus HR-II; Wako Pure Chemical Industries, Ltd.), and total calcium was measured by the orthocresolphthalein complexone
method (Serotec Ca-AL; Serotec Company).
Calcium levels were determined by colorimetry through a reaction with o-cresolphthalein complexone
. ALP activity was measured using an ALP Colorimetric assay kit, in accordance with the kit's instructions.
The samples were analyzed for serum total calcium, by the O-Cresolphthalein complexone
method  and for serum sodium and potassium, by the ion selective electrode method , bicarbonate by blood gas analyzer and blood urea and creatinine using Urease and Jaffe's method respectively [9,10] The data was collected and analyzed using SPSS 11.5 version.
To detect calcium concentrations in culture medium, we used a calcium concentration assay kit (o-cresolphthalein complexone
kit; Teco Diagnostics, Anaheim, CA, USA) following the manufacturer's instructions.