AOSLO images were acquired confocally
, using a standardized protocol that obtained images focused at the following planes: the lesion of interest, posteriorly at the photoreceptor level and anteriorly at the nerve fiber layer.
a) Raster-scan based photoacoustic microscopy: By using a single focused ultrasonic transducer, usually placed confocally
with the irradiation laser beam, PAM forms a 1D image at each position, where the flight time of the ultrasound signal provides depth information.
This allows very rough or heavily inclined samples to be chemically characterized precisely, automatically and easily, while also being confocally