Traditionally, SAMs have been evaluated according to their adhesive function, assessed typically via their ability to aggregate cells in cell-based assays, copurify
with synaptosomal membrane fractions, and localize to synapses in electron microscopy images.
It has been demonstrated that polymer precipitation of exosomes can result in contamination by protein aggregates that copurify
with nanovesicles ; in particular, the biophysical properties of immune complexes overlap with microvesicles, interfering with the interpretation of ensuing results .
Garlid, "Mitochondrial PKC[epsilon] and mitochondrial ATP-sensitive [K.sup.+] channel copurify
and coreconstitute to form a functioning signaling module in proteoliposomes," Circulation Research, vol.
Those proteins that copurify
with the Buchnera cells through the enrichment series are predicted to be associated with the Buchnera cells (Fig.
Mitochondrial PKC epsilon and mitochondrial ATP-sensitive 1< + channel copurify
and coreconsti-tute to form a functioning signaling module in proteoliposomes.
Mitochondrial PKC epsilon and mitochondrial ATP-sensitive K+ channel copurify
and coreconstitute to form a functioning signaling module in proteoliposomes.
The RNase cocktail enzyme mix was necessary because the Qiagen[R] spin columns copurify
RNA and DNA in parallel when both are present in a sample.
coli recombinant proteins, that copurify
with MSP5, interfere with the ELISA giving rise to false positives.
Internal amplification controls (IACs) that copurify
and coamplify with the target nucleic acid are sensitive indicators of loss or degradation of the target during processing and inhibition of amplification and detection.
Dyes present in the commercial mouthwashes and remaining in this supernatant fraction could copurify
with the DNA and potentially interfere with downstream analyses.
"Fibrinogen is isolated from plasma by a method that tends to copurify
with a lot of viruses," notes Drohan.
It is well established that most kinases (proline-directed and non-proline-directed) copurify
with their cytoskeletal substrates as multimeric complexes.