counterstain

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coun·ter·stain

 (koun′tər-stān′)
n.
A stain of a contrasting color used to color the components in a microscopic specimen that are not made visible by the principal stain.
tr.v. coun·ter·stained, coun·ter·stain·ing, coun·ter·stains
To color (a microscopic specimen) with such a stain.

counterstain

(ˈkaʊntəˌsteɪn)
vb
1. (Biology) to apply two or more stains in sequence to (a specimen to be examined), each of which colours a different tissue
2. (Biology) (tr; usually passive) to apply (one of a series of stains) to a specimen to be examined: haematoxylin is counterstained with eosin.

coun•ter•stain

(ˈkaʊn tərˌsteɪn)

n.
1. a second stain applied to a microscopic specimen for contrast.
v.t.
2. to treat (a microscopic specimen) with a counterstain.
v.i.
3. to become counterstained; take a counterstain.
[1890–95]

counterstain


Past participle: counterstained
Gerund: counterstaining

Imperative
counterstain
counterstain
Present
I counterstain
you counterstain
he/she/it counterstains
we counterstain
you counterstain
they counterstain
Preterite
I counterstained
you counterstained
he/she/it counterstained
we counterstained
you counterstained
they counterstained
Present Continuous
I am counterstaining
you are counterstaining
he/she/it is counterstaining
we are counterstaining
you are counterstaining
they are counterstaining
Present Perfect
I have counterstained
you have counterstained
he/she/it has counterstained
we have counterstained
you have counterstained
they have counterstained
Past Continuous
I was counterstaining
you were counterstaining
he/she/it was counterstaining
we were counterstaining
you were counterstaining
they were counterstaining
Past Perfect
I had counterstained
you had counterstained
he/she/it had counterstained
we had counterstained
you had counterstained
they had counterstained
Future
I will counterstain
you will counterstain
he/she/it will counterstain
we will counterstain
you will counterstain
they will counterstain
Future Perfect
I will have counterstained
you will have counterstained
he/she/it will have counterstained
we will have counterstained
you will have counterstained
they will have counterstained
Future Continuous
I will be counterstaining
you will be counterstaining
he/she/it will be counterstaining
we will be counterstaining
you will be counterstaining
they will be counterstaining
Present Perfect Continuous
I have been counterstaining
you have been counterstaining
he/she/it has been counterstaining
we have been counterstaining
you have been counterstaining
they have been counterstaining
Future Perfect Continuous
I will have been counterstaining
you will have been counterstaining
he/she/it will have been counterstaining
we will have been counterstaining
you will have been counterstaining
they will have been counterstaining
Past Perfect Continuous
I had been counterstaining
you had been counterstaining
he/she/it had been counterstaining
we had been counterstaining
you had been counterstaining
they had been counterstaining
Conditional
I would counterstain
you would counterstain
he/she/it would counterstain
we would counterstain
you would counterstain
they would counterstain
Past Conditional
I would have counterstained
you would have counterstained
he/she/it would have counterstained
we would have counterstained
you would have counterstained
they would have counterstained
ThesaurusAntonymsRelated WordsSynonymsLegend:
Noun1.counterstain - a stain of contrasting color that is used when the principal stain does not show the structure clearly
stain - (microscopy) a dye or other coloring material that is used in microscopy to make structures visible
References in periodicals archive ?
Sections were washed in PBS and subsequently counterstained using DAPI (Haase et al.
The slides were counterstained for 1 minute with hematoxylin and then dehydrated with sequential ethanol for sealing and microscope observation.
staining was performed with bond polymer detection kit and ultra view 3,3'-diaminobenzidine (DAB) detection kit and the sections were developed with diaminobenzidine tetrahydrochloride and counterstained with hematoxylin.
Subsequently, sections were incubated with 3,3'-diaminobenzidine (DAB) and counterstained with hematoxylin (Mayer).
After washing the slides with tap water, it was counterstained with 0.
The specimens were then counterstained with haematoxylin for 8 minutes and post-counterstained with bluing reagent for four minutes.
The slides were counterstained with Harris hematoxylin for 10 seconds, then washed with distilled water and dried in alcohol.
For the nuclear staining, colonies were counterstained with Hochest33342 (Life Technology, 62249, NY, NY, USA) for 5 min, washed three times with PBS for 5 min each, and analyzed under fluorescence microscope (N-STORM, Nikon).
Finally, sections were counterstained with DAPI and mounted with Vectashield (Vector Labs).
Then, samples were counterstained with Harris hematoxylin for 30 seconds at room temperature, followed by dehydration and slide mounting with synthetic resin.