counterstain

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coun·ter·stain

 (koun′tər-stān′)
n.
A stain of a contrasting color used to color the components in a microscopic specimen that are not made visible by the principal stain.
tr.v. coun·ter·stained, coun·ter·stain·ing, coun·ter·stains
To color (a microscopic specimen) with such a stain.
American Heritage® Dictionary of the English Language, Fifth Edition. Copyright © 2016 by Houghton Mifflin Harcourt Publishing Company. Published by Houghton Mifflin Harcourt Publishing Company. All rights reserved.

counterstain

(ˈkaʊntəˌsteɪn)
vb
1. (Biology) to apply two or more stains in sequence to (a specimen to be examined), each of which colours a different tissue
2. (Biology) (tr; usually passive) to apply (one of a series of stains) to a specimen to be examined: haematoxylin is counterstained with eosin.
Collins English Dictionary – Complete and Unabridged, 12th Edition 2014 © HarperCollins Publishers 1991, 1994, 1998, 2000, 2003, 2006, 2007, 2009, 2011, 2014

coun•ter•stain

(ˈkaʊn tərˌsteɪn)

n.
1. a second stain applied to a microscopic specimen for contrast.
v.t.
2. to treat (a microscopic specimen) with a counterstain.
v.i.
3. to become counterstained; take a counterstain.
[1890–95]
Random House Kernerman Webster's College Dictionary, © 2010 K Dictionaries Ltd. Copyright 2005, 1997, 1991 by Random House, Inc. All rights reserved.

counterstain


Past participle: counterstained
Gerund: counterstaining

Imperative
counterstain
counterstain
Present
I counterstain
you counterstain
he/she/it counterstains
we counterstain
you counterstain
they counterstain
Preterite
I counterstained
you counterstained
he/she/it counterstained
we counterstained
you counterstained
they counterstained
Present Continuous
I am counterstaining
you are counterstaining
he/she/it is counterstaining
we are counterstaining
you are counterstaining
they are counterstaining
Present Perfect
I have counterstained
you have counterstained
he/she/it has counterstained
we have counterstained
you have counterstained
they have counterstained
Past Continuous
I was counterstaining
you were counterstaining
he/she/it was counterstaining
we were counterstaining
you were counterstaining
they were counterstaining
Past Perfect
I had counterstained
you had counterstained
he/she/it had counterstained
we had counterstained
you had counterstained
they had counterstained
Future
I will counterstain
you will counterstain
he/she/it will counterstain
we will counterstain
you will counterstain
they will counterstain
Future Perfect
I will have counterstained
you will have counterstained
he/she/it will have counterstained
we will have counterstained
you will have counterstained
they will have counterstained
Future Continuous
I will be counterstaining
you will be counterstaining
he/she/it will be counterstaining
we will be counterstaining
you will be counterstaining
they will be counterstaining
Present Perfect Continuous
I have been counterstaining
you have been counterstaining
he/she/it has been counterstaining
we have been counterstaining
you have been counterstaining
they have been counterstaining
Future Perfect Continuous
I will have been counterstaining
you will have been counterstaining
he/she/it will have been counterstaining
we will have been counterstaining
you will have been counterstaining
they will have been counterstaining
Past Perfect Continuous
I had been counterstaining
you had been counterstaining
he/she/it had been counterstaining
we had been counterstaining
you had been counterstaining
they had been counterstaining
Conditional
I would counterstain
you would counterstain
he/she/it would counterstain
we would counterstain
you would counterstain
they would counterstain
Past Conditional
I would have counterstained
you would have counterstained
he/she/it would have counterstained
we would have counterstained
you would have counterstained
they would have counterstained
Collins English Verb Tables © HarperCollins Publishers 2011
ThesaurusAntonymsRelated WordsSynonymsLegend:
Noun1.counterstain - a stain of contrasting color that is used when the principal stain does not show the structure clearly
stain - (microscopy) a dye or other coloring material that is used in microscopy to make structures visible
Based on WordNet 3.0, Farlex clipart collection. © 2003-2012 Princeton University, Farlex Inc.
References in periodicals archive ?
After three washes with PBST, cells were incubated with Alexa Fluor[R] 555 Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed secondary antibody (#A31572, Invitrogen; 1:500 in PBST) for 1 h in the dark at room temperature, then washed three times in PBST and counterstained with DAPI for 10 min at room temperature.
After washing in PBS, the nuclei were counterstained with Hoechst 33258 (1:100,000 diluted in 0.1% Triton-X in distilled water, Sigma-Aldrich, St.
Finally washed in running tap water and were counterstained with haematoxylin, dehydrated and mounted and observed under microscope.
Frozen sections were incubated with the rabbit anti-AvBD8 primary antibody, followed by incubation with the Alexa Fluor 488-conjugated goat anti-rabbit IgG secondary antibody, and were counterstained with 4',6-diamidino-2-phenylindole.
Sections were counterstained with Mayer's hematoxylin for 30s, dehydrated, covered with coverslip and examined by light microscopy.
Finally, sections were counterstained with 5 [micro]g/ml 4', 6-diamidino-2-phenylindole (Beyotime, Jiangsu, China) for 10 min in the dark at room temperature and mounted with antifade mounting medium (Beyotime, Jiangsu, China).
Ultrathin sections were sliced and mounted on a copper grid and counterstained with uranyl acetate and lead citrate.
Nuclei were counterstained with haematoxylin, and the sections were rehydrated, cleared in xylene, and mounted with Pertex.
Cell nuclei were counterstained with 4/,6-diamidino-2-phenylindole (DAPI).
After this procedure, the slides were thoroughly rinsed in distilled water, toned in 0.2% gold chloride for 1 min, rinsed in distilled water, treated with 5% sodium thiosulfate for 5 minutes, rinsed in distilled water, and counterstained with nuclear red fast solution for 2 min.
All sections were counterstained with 4',6-diamidino-2-phenylindole (DAPI).
For immunofluorescence observations, cross-presented OVA was stained with red (25-D1.16), EEA1 and Rab7 are all stained with green, and nuclei were counterstained with blue (DAPI).