cremaster

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cremaster

(krɪˈmæstə)
n
the muscle which raises and lowers the testiclesthe hook-like protuberance at the end of a chrysalis from which it hangs
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To elucidate the role of RAGE and ICAM-1 in the context of PC-induced inhibition of leukocyte recruitment, leukocyte adhesion in postcapillary venules of surgically prepared cremaster muscles of wild-type (WT), [RAGE.sup.-/-], and [Icam-1.sup.-/-] mice was observed using intravital microscopy.
For this purpose, Giemsa staining of whole mounts of fMLP-stimulated cremaster muscles, obtained after the respective intravital microscopic experiment, was performed (Figure 1(c)).
Interestingly, in vivo experiments using the models of trauma- and TNF[alpha]-induced inflammation of murine cremaster muscles revealed that RAGE and ICAM-1 act together in mediating leukocyte adhesion in a stimulus-dependent manner [5, 6].
The cremaster muscles are the muscles that control the height of the testicles, which usually varies with changes in temperature.
To this end, superfusion of murine cremaster muscles with SB203580 (100 nM) for 30 min prior to and for 1 h following the placement of KC gel did not significantly modify the rolling flux and rolling velocity of neutrophils as compared to the control saline-superfused cremaster muscle (P > 0.05; Figures 1(a) and 1(b)).
To analyse the subsequent steps of neutrophil recruitment in the same cremaster muscles, we further quantitatively-visualized and determined the number of adherent and emigrated neutrophils.
The mouse cremaster muscle preparation was used to study neutrophil behaviour in microcirculation and tissue as described previously [24-26].