The crucian carps (10-12cm) were collected for the heat stress experiment.
But the research about Wap65 in crucian carp is very limited.
CcWap65 was significantly up-regulated in the liver of crucian carp after heat stress, suggesting that increase in Wap65 gene may be related to high water temperature stress and play important roles in high water temperature environment of crucian carp.
In this study, we report the cloning and characterization of the coding sequense (CDS) encoding full sequence of a Wap65 protein in the crucian carp (Carassius carassius).
Isolation of total RNA, synthesis of first strand cDNA and cloning of the crucian carp Wap65-1
To clone the CDS of crucian carp Wap65 (CcWap65), a pair of primers WF and WR (Table I) were designed according to the conserved regions of goldfish (GenBank: D50437.
The 96 h lethal concentrations of Pb2+ for Crucian carps are summarized in Table 3 with value 29.
This study evaluated the acute toxicity effects of Pb2+ on essential trace metals behavior and histopathology of Crucian carp (Carassius auratus gibelio).
Crucian carp is one of the famous food fish in China and in other parts of the world.
However the knowledge pertaining to acute toxicity effects and histopathological alteration due to Pb exposure in Crucian carp (Carassius auratus gibelio) is limiting.
In the present study we determined the 96 h median lethal concentration (LC50) of Pb2+ and examined the histopathological changes in liver kidney gill and brain of Crucian carp (Carassius auratus gibelio).
Aquarium water quality and Pb2+ exposure: Crucian carp (Carassius auratus gibelio) were used as model animal for this study.