Over the past 30 years, techniques for cryopreservations of human embryos, oocytes, and ovarian tissues have been developed, with two main techniques commonly being used: the traditional, slow-freezing method, and vitrification, a novel technique combining ultra-rapid cooling time with high cryoprotectant concentration, engendering glass-like formation to avoid damaging ice crystals.
Studies provided reassuring data on safety and efficacy of oocyte and embryo cryopreservations, and both embryo and oocyte vitrifications are excellent options for female patients to reserve reproductive fertility.
Objective: To evaluate the safety and risk of cryopreservation in female fertility preservation.
Numerous individual studies investigating the different reproductive outcomes of cryopreservation on human embryos, immature or mature oocytes, or ovarian tissues have been reported.
sup] This technique has been investigated by a respectable number of research teams, and hereby we summarized the latest systematic studies to discuss the clinical effects of cryopreservation on human embryos.
Mature oocyte cryopreservation is a freezing method, which was just approved by ASRM in 2012.
Cryopreservations on eggs, embryos, and larvae have been tried in C.
DMSO and PG have successfully been used in cryopreservations of embryos and trochophore larvae in C.
KEY WORDS: cryopreservation, Saccostrea glomerata, larvae, DMSO, PG, oyster
The cryopreservation of gametes and embryos has been studied for nearly six decades since Polge et al.
It is anticipated that the development of cryopreservation techniques would further enhance the capacity and efficiency of the program.
When the freezing protocol with the seeding step and 10% DMSO were used in the cryopreservation, the postthaw survival rates were not affected significantly (P = 0.