Composition of cryoprotective
solution used to freeze sperm of A.
Before cryopreservation can be realized, evidence from numerous studies on fish and other species of bivalve mollusc has highlighted the importance of identifying the type and concentration of appropriate sperm cryoprotective
agents (CPAs) for the species of interest, as many CPAs show varying degrees of toxicity to sperm either in isolation, or when applied in different combinations (Chao et al.
It maintains integrity of the skin under freezing conditions, showing nigh cryoprotective
Additionally, the effect of fall harvest management on cryoprotective
sugars including sucrose and RFO has not been investigated.
An equal amount of cryoprotective
additive solution (28% glycerol, 3% mannitol, 0.65% NaCl) was added to packed erythrocytes and thoroughly mixed.
After addition of the cryoprotective
solution, the unit may be frozen in the liquid or vapor phase of nitrogen, or an ultra-cold mechanical freezer.
They also add cryoprotective
agents such as glycerol, which are thought to act as antifreeze solutions and which may protect cell parts by stabilizing membranes and proteins.
Newton and Duman  expressed osmotin-like cryoprotective
protein from S.
Briefly, E14.5 embryos were removed and their brains were dissected and fixed in 4% PFA overnight and then transferred to a cryoprotective
solution (30% sucrose in PBS).
Effects of bovine sperm cryopreservation using different freezing techniques and cryoprotective
agents on plasma, acrosomal and mitochondrial membranes.
Effect of cryoprotective
additives and cryopreservation protocol on sperm membrane lipid peroxidation and recovery of motile human sperm.