There was no clear evidence of any differences as a result of using (a) cycloheximide
vs puromycin treatment for NMD inhibition; (b) differing RNA extraction methods; (c) oligo d(T) and random hexamers vs gene specific primers; (e) various methods of DNase treatment; and (f) a particular type or brand of Taq polymerase.
Effect of cycloheximide
on postharvest performance in cut spikes of Consolida q]acis cv.
1973, "Effect of lactose, ethanol and cycloheximide
on the translocation pattern of radioactive compounds and sclerotium formation in Sclerotium rolfsii," J.
Metallothionein synthesis probably occurs through formation of a specific mRNA because both cycloheximide
and actinomycin D, administered concomitantly with cadmium, block the incorporation of [[sup.
Therefore, most bacteria grown on FMA should have been able to digest keratin and were therefore, considered keratin degrading, Both media contained 100 [mu]g/ml of cycloheximide
to inhibit fungal growth (Smit et al.
In developing the medium, researchers used cycloheximide
MEI agar contains nalidixic acid, sodium azide, triphenyl tetrazolium chloride, and cycloheximide
to inhibit the growth of other microorganisms (12, 14, 15).
Starting at 24 hours or 84 hours post-insemination (hpi) embryos were transferred to culture drops containing 25 [micro]g/ml alpha-amanitin or 2 [micro]g/ml cycloheximide
for a 60-hour exposure.
In this new study, designed to investigate the mechanisms involved in the modulation of cell viability of the human androgen-dependent prostate cancer, LNCaP cells lines were incubated with either enterolactone (70-100 microMols) or hydroxymatairesinol (70-100 microMols) for 48 hours, with estradiol (100 microMols) and the cytotoxic agent cycloheximide
(10 microMols) used as the experimental controls.
2 percent malt extract agar amended with 250 [micro]g/ml of streptomycin sulphate and 100 [micro]g/ml of cycloheximide
2,13) Biochemical characteristics that help to further differentiate B capitatus include the inability to use potassium nitrate as a sole nitrogen source, the inability to hydrolyze urea, and resistance to cycloheximide
Findings showed microsomal membrane-prepared fractions from HaCaT cell cultures, with 0, 1, 2 and 4 ug/well of cycloheximide
, solubilized synthetic melanin.