Afterward, 1 mL of the suspension was added to a liquid medium with yeast extract (1%), peptone (4%), glucose (6%), calcium carbonate (1%), 100 mg/L of cycloheximide
and 150 mg/L of penicillin at a pH 6.6.
(seaweed) penicillin + streptomycin Mytilus edulis (mollusk) penicillin + streptomycin + chloramphenicol Tetraselmis maculata (phytoplankton) + Tween-80 cycloheximide
Crassostrea virginica (mollusk) penicillin + streptomycin Euterpina acutifrons, Temora turbinata, Oncaea sp.
The purpose of this article is to provide an insight into the share of dermatophytes, yeasts and non-der-matophyte molds as potential pathogens in OM, grown on Sabouraud agar without cycloheximide
, as well as the prevalence of positive and negative results of DME, and grown isolates, according to patient sex and age.
In this study, the diagnosis of dermatophytosis was made by demonstrating dermatophytic hyphae under microscope by KOH mount and culturing the specimen on SDA with and without cycloheximide
They were incubated at 37[degrees]C in Krebs-Ringer bicarbonate buffer, pH 7.4, equilibrated with 95% [O.sub.2] and 5% C[O.sub.2] containing glucose (5 mM) in the presence of cycloheximide
(0.5 mM) to prevent protein synthesis and the reincorporation of tyrosine back into proteins.
Nutrient agar with cycloheximide
(0.5 mg/ml to suppress fungal growth)  and Martin's Rose Bengal agar medium strips were used for the isolation of bacteria and fungi, respectively.
Hair and scab samples were cultured in Petri dishes containing Sabouraud Dextrose Agar (SDA) with yeast extract, chloramphenicol and cycloheximide
. Petri dishes were incubated aerobically at 25[degrees]C and examined daily for five weeks.
Time-dependent effects of cycloheximide
on long-term memory in the cuttlefish.
The specimens were cultured in 2 plates/tubes, one containing Sabouraud-chloramphenicol dextrose agar and the other containing Sabouraud-chloramphenicol cycloheximide
. Cultures were incubated at 22-27[degrees]C and evaluated for growth after 48 h and then once weekly for a month.
The measurement of half-life of the protein was done using the cycloheximide
chase assay based on the method described earlier .
Subsequently, the CSF, inoculated onto Sabouraud-chloramphenicol media without cycloheximide
, incubated at 30[degrees]C, yielded smooth yeast colonies later confirmed as Cryptococcus neoformans var.
For the reagent treatments, primary cultures or cell lines were treated with 10 [micro]M MG132 (Sigma), 1 [micro]g/ml cycloheximide
(CHX) (Sigma), and 50 [micro]M chloroquine (CQ) (Sigma).