B (100 [micro]L at final concentration: 6 [micro]L/mL) was added after 44 h of culture.
B at final concentration of 6ug/ml was added to 44th hour of the culture of lymphocytes, according to the method of Fenech and Morley.
The FIBTEM test is similar to the EXTEM test, but the platelet effect on clotting is completely inhibited by cytochalasin
D and therefore measures the fibrinogen/fibrin contribution to clotting.
Polyploidy induced in the American oyster, Crassostrea virginica, with cytochalasin
B was purchased from Sigma Chemicals Co., USA.
After this period, aliquots (500 [micro]L) were withdraw to perform the Comet Assay and the remaining cell suspension was concentrated by centrifugation (1000 rpm / 5 minutes), the supernatant discarded, and 5 mL of complete culture medium added with Cytochalasin
B in concentration of 5.0 [micro]g / mL culture medium.
At the 44th hour of cultivation, cytochalasin
B was added to the cell culture at a concentration of 6 [micro]g/mL in order to stop cytokinesis.
These cells were also shown to be sensitive to another blocker of glucose transporters, cytochalasin
B, thus suggesting that the mechanism by which phloretin induces cell death in senescent cells is related to their increased metabolic requirement.
The disruption of cytoskeleton by cytochalasin
, colchicine, and nocodazole or the disruption of Golgi apparatus by monensin and brefeldin A just slightly inhibits cholesterol transport from ER to PM [4, 95].
Vasiliev, "Disruption of actin microfilaments by cytochalasin
D leads to activation of p53," FEBS Letters, vol.
In this study, we investigated the effect of TGF[beta]-induced actin cytoskeleton modifications on the potential of hMSCs to differentiate into osteogenic and adipogenic lineages, as well as the effect of the actin polymerization inhibitor cytochalasin
D, an inhibitor of F-actin polymerization, blocks the late phase of LTP but not the early phase .