Poliovirus/PVSRIPO causes drastic, rapid cytopathogenic
effects leading to gross morphologic changes and cell detachment.
We infected cells in duplicate with 200 [mciro]L of stool extract, incubated at 36[degrees]C, and observed microscopically for cytopathogenic
effect (CPE) daily for 5 days.
Viruses from the culture supernatants of 293A cells that showed cytopathogenic
effects were purified by cesium chloride banding.
The analyses of the plaques (plaque-forming unit, PFU) and the cytopathogenic
effect (CPE) were performed 3-6 days later.
The IBH-HPS virus was successfully adapted after three blind passages (Vero cell line) and the 4th passage was fully adapted as it gave cytopathogenic
effect (CPE) in 48 h post infection in Vero cells.
The cells were then cultured for 7-10 days to induce cytopathogenic
The influenza H1N1 was found to induce severe cytopathogenic
effects in a dose-dependant manner, however the cells treated with RGE displayed decreased viral cytopathogenic
effects and reduced cell death caused by the virus.
effect of poliomyelitis viruses in vitro on, human embryonic tissues.
The virus infected flasks were incubated at 37 C and 5 % CO2 examined under inverted microscope after every 24 hours to check cell monolayer and detect any cytopathogenic
Viruses contaminationCells were observed by phase-contrast microscopy for cytopathogenic
examination, operation in details was similar to Li et al.
The effects of the combination were compared to several positive controls (acyclovir, ribavirin and amantadine hydrochloride) and assessment of the effects was done by standard viral viability tests (plaque reduction assay, cytopathogenic
assays, virus titrations, analysis of the viral proteins in virus-specific enzyme immunoassays, and haemagglutination tests).
Determination of virus-induced cytopathogenic
effects and virus titres revealed that EPs[R] 7630 at concentrations up to 100 [micro]g/ml interfered with replication of seasonal influenza A virus strains (H1N1, H3N2), respiratory syncytial virus, human coronavirus, parainfluenza virus, and coxsackie virus but did not affect replication of highly pathogenic avian influenza A virus (H5N1), adenovirus, or rhinovirus.