dGTP


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Related to dGTP: dTTP, dATP

dGTP

 (dē′jē′tē′pē′)
n.
One of the two purine nucleotides that are used to synthesize DNA.

[d(eoxy)- + g(uanosine) + t(ri)p(hosphate).]
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DGTP is to act as a link connecting all hoteliers in the emirate - providing learning opportunities and facilitating the implementation of initiatives.
For codons 12, 13, and 14 of wild-type KRAS and 12b KRAS mutant (G [right arrow] A), both molecules are elongated when dGTP is dispensed, but the nascent strands are out of phase because the strand replicating the wild-type allele incorporates 2 dGTPs, whereas the one replicating the mutant allele only incorporates one dGTP (Figures 5, A, and 7, A).
Se uso la MASTER MIX como mezcla de PCR (400 [micron]M dATP, 400 [micron]M dGTP, 400 [micron]M dCTP, 400 [micron]M dTTP), MgCl 2, (3mM), Taq polimerasa 5 U/ul (8).
5 mM each of dATP, dCTP, dGTP and dTTP), 1 of 10 pmol of each primer and 1 U HS Prime Taq (GeNet Bio, Korea) in a 25 [micro]l reaction volume.
2002); 5[micro]l of a dNTP mixture containing 1 mM each of dATP, dCTP, dGTP, and dTTP; 2[micro]l of a 25-mM solution of magnesium chloride; 0.
5 [micro]M of each primer, 100 [micro]M of each deoxynucleotide trifosfate (dATP, dGTP, dCTP, dTTP) and 1.
Labeling was obtained using a PCR procedure with the following dNTP concentrations: 200 [micro]M dATP, 200 [micor]M dCTP, 200 [micro]M dGTP, 150 [micro]M dTTP, and 50 [micro]M dig-11-dUTP (Roche Molecular Biochemicals).
Polymerase Chain Reactions (PCR) were carried out in a final volume of 25 [micro]l containing 20 ng template DNA, 100[micro]M each dATP, dTTP, dGTP & dCTP, 20 ng of decanucleotide primers, 1.
For PCR amplification of the CYP3A43 rs501275 polymorphism we used the primers F 5'-GTCAATGGCAATTTTCTGTT-3' and R 5'-CTGTCTTCACAAACCAGATG-3' in a 50 [micro]l reaction mix containing 50 ng of genomic DNA, 120 ng of each of the forward (F) and reverse (R) primers, 200 [micro]M of dATP, dCTP, dGTP and dTTP, 1.