Tenofovir disoproxil fumarate (TDF) is a prodrug of tenofovir diphosphate, a structural analog of deoxyadenosine
triphosphate, which is the natural substrate for the viral enzyme reverse transcriptase.
TA cloning is achieved by Taq DNA polymerase which has non-template-dependent terminal transferase activity which helps adding a single deoxyadenosine
(A) to 3' ends of PCR products.
Its main function is to catalyze the conversion process of adenosine to inosine and deoxyadenosine
to deoxyinosine and ammonia is released in this process.
Adenosine deaminase (ADA) is an enzyme which is involved in purine metabolism in which it is responsible for conversation of adenosine and deoxyadenosine
to inosine and deoxyinosine respectively resulting in release of ammonia.
It is now being considered for use in GBM therapy.2,3 The positive interaction between gemcitabine and radiotherapy is likely due to a combination of mechanisms that include deoxyadenosine
triphosphate depletion, cell cycle redistribution and inhibition of DNA synthases and repair.2Gemcitabine can be deaminated to its metabolite difluorodeoxyuridine (dFdU), also a radiosensitizer.3
Bare et al., "Sensitive GCMS/MS method to measure deuterium labeled deoxyadenosine
in DNA from limited mouse cell populations," Analytical Chemistry, vol.
Cordycepin (37 deoxyadenosine
) is one of the bioactive components isolated from Cordyceps militaris.
Cytochrome c binds to Apaf-1 in the presence of dATP/ATP (deoxyadenosine
triphosphate/adenosine triphosphate), which then binds to procaspase-9 to form a cytochrome c-Apaf-1-Caspase-9 complex, called apoptosome.
MDA reacts with deoxyadenosine
and deoxyguanosine in DNA, forming DNA MDA and other thiobarbituric acid reactive substances (TBARS) condense with two equivalents of thiobarbituric acid to give a fluorescent red derivative.
TK is a key enzyme in the pyrimidine salvage pathway, catalyzing the transfer of the terminal phosphate from adenosine triphosphate to the 5'-hydroxyl group of thymidine, producing deoxyadenosine
triphosphate, which is subsequently incorporated into DNA.
This enzyme irreversibly converts adenosine to inosine and deoxyadenosine
to deoxyinosine, and the speed rate of this reaction gives an account of enzymatic activity, calculated as ADA concentration in the laboratory.
The SNP allele identification (5 [micro]L) was 1 [micro]L digested PCR product; 0.01-0.6 [micro]M of each primer (Supplementary Table S1); 3.5 mM MgC[L.sub.2], 1 x Thermo Sequenase DNA polymerase buffer; 0.5 [micro]M fluorescein-labeled 2,3-dideoxycytidine-5' triphosphate (ddCTP) (PerkinElmer Life and Analytical Sciences, USA); 0.5 [micro]M each unlabeled deoxyguanosine triphosphate (dGTP), deoxythymidine triphosphate (dTTP), and deoxyadenosine
triphosphate (dATP); and 1 U Thermo Sequenase DNA Polymerase (GE Healthcare, UK).