Robertson, "Anti-streptococcal antibodies in the diagnosis of acute and post-streptococcal disease: streptokinase versus streptolysin O and deoxyribonuclease
B," Pathology, vol.
Lee, "Intrapleural tissue plasminogen activator and deoxyribonuclease
therapy for pleural infection," Journal of Thoracic Disease, vol.
Scherz et al., "Coronary neutrophil extracellular trap burden and deoxyribonuclease
activity in ST-elevation acute coronary syndrome are predictors of st-segment resolution and infarct size," Circulation Research, vol.
In addition, deoxyribonuclease
with a final concentration of 140U/mL in magnesium peptone water buffer (0.1% peptone and 5 mM Mg[Cl.sub.2]), which was incubated at 37[degrees]C for 16 h, and proteinase K with a final concentration of 100 [micro]g/mL in Tris-peptone buffer (0.1% peptone, 20 mM Tris-HCl [pH 7.5], and 100 mM NaCl), which was incubated at 37[degrees]C for 16 h, were added successively to the established biofilm in MWP.
Finally, RNAs were treated by Deoxyribonuclease
I (Thermo, USA) to remove contaminating genomic DNA.
It could initiate a molecular cascade that culminates in cell apoptosis , including promoting the depolarization of the mitochondria, then releasing second mitochondria-derived activator of caspases (Smac) and cytochrome c (cyt c) to the cytoplasm, enhancing caspase 3, and finally activating deoxyribonuclease
(DNase) to degrade DNA of the neurons [6, 8].
After growth, staphylococci were identified on the basis of colony characteristics, Gram staining, pigment production, hemolytic and the following biochemical reactions: catalyses activity, coagulated test (rabbit plasma), Oxidase test, glucose O/F test, resistance to bacitracin (0.04 U), mannitol fermentation on Mannitol Salt Agar (MSA) (Merck, Darmstadt, Germany), urease activity, nitrate reduction, novobiocin resistance, phosphatase, deoxyribonuclease
(DNase) test and carbohydrate (xylose, sucrose, trehalose and maltose, fructose, lactose, mannose) fermentation test (12).
Recombinant human deoxyribonuclease
(rhDNAse (Pulmozyme))  and glucocorticoids  are not recommended in the management of infants and children with bronchiolitis.
Total RNA was isolated from leave tissues of 200 mg, treated with the deoxyribonuclease
I to remove any DNA contamination, was reversely transcribed using Plant RNA Mini Kit (Watson Biotechnologies, Inc, China), DNase I Kit (Promega, Madison, WI, USA), or PrimeScript(r) RT Master Mix Perfect Real Time Kit (TaKaRa, China), respectively.
Such pathogenicity is associated with the lethal extracellular toxin which has defined as enzyme activity as collagenas, hyalauronidase and deoxyribonuclease
Currently, there is convincing evidence of the existence of deoxyribonuclease
involved in the apoptotic process .
cDNA was synthesized from each RNA preparation using M-MLV reverse transcriptase (Invitrogen), and the total RNA (500 ng) was treated with 0.5 U of deoxyribonuclease
I (DNase I; Invitrogen) to digest genomic DNA.