Structure and function of cellular deoxyribonucleoside
By (a) synthesizing libraries of non-natural nucleosides ( reporter probes ) to generate artificially colored CEST contrast, and (b) performing directed evolution of deoxyribonucleoside
kinase (dNK) enzymes ( reporter genes ) to phosphorylate those nucleosides, the multicolor genetically encoded MRI reporter system will be created.
2 mM deoxyribonucleoside
triphosphates, 1x reaction buffer, 2mM Mg[Cl.
2]) (YeaTaq, Taiwan), 4 [micro]L of deoxyribonucleoside
Then, 4 [micro]l of reaction buffer, 1 [micro]l of RiboLock RNase inhibitor, 2 [micro]l of deoxyribonucleoside
triphosphate, and 1 [micro]l of Moloney Murine Leukemia Virus Reverse Transcriptase were added to the solution until its final volume reached 20 [micro]l.
1) converts ribonucleoside diphosphates into deoxyribonucleoside
diphosphates being essential for DNA replication, which is why the enzyme is considered an excellent target for anticancer therapy.
5 [micro]L of dNTP mix (25 [micro]M of each deoxyribonucleoside
triphosphate - dATP, dCTP, dGTP and dTTP) (Invitrogen, Carlsbad, CA, USA), 1.
Toward single molecule DNA sequencing: direct identification of ribonucleoside and deoxyribonucleoside
5'-monophosphates by using an engineered protein nanopore equipped with a molecular adapter.
165 [micro]l in 20 [micro]l of reaction volume) of each deoxyribonucleoside
triphosphate (dATP, dTTP, dGTP, dCTP) and 1.
5 mM MgCl2 200 M of each deoxyribonucleoside
triphosphate and 0.
0, 200uM of deoxyribonucleoside
triphosphate (dNTPs), 100 pmoles of each forward and reverse primer, 2.
2]; 1 [micro]L of deoxyribonucleoside
triphosphates (2 mM each dATP, dCTP, dTTP, and dGTP); 0.