1) converts ribonucleoside diphosphates into deoxyribonucleoside
diphosphates being essential for DNA replication, which is why the enzyme is considered an excellent target for anticancer therapy.
Then, 4 [micro]l of reaction buffer, 1 [micro]l of RiboLock RNase inhibitor, 2 [micro]l of deoxyribonucleoside
triphosphate, and 1 [micro]l of Moloney Murine Leukemia Virus Reverse Transcriptase were added to the solution until its final volume reached 20 [micro]l.
165 [micro]l in 20 [micro]l of reaction volume) of each deoxyribonucleoside
triphosphate (dATP, dTTP, dGTP, dCTP) and 1.
0 [micro]M of each deoxyribonucleoside
A reaction mixture of 50 pL contained 1 mM deoxyribonucleoside
triphosphate, 2 mM magnesium chloride, 200 nM primers, 1.
2]; 1 [micro]L of deoxyribonucleoside
triphosphates (2 mM each dATP, dCTP, dTTP, and dGTP); 0.
An Escherichiacoli system expressing human deoxyribonucleoside
salvage enzymes for evaluation of potential antiproliferative nucleoside analogs.
113 mmol/L deoxyribonucleoside
triphosphates (dNTPs), 0.
2 mM solution of deoxyribonucleoside
94 from mitochondria Deoxyribonucleoside
monophosphate 0 3.
2], 50 mmol/L KCl, 1 unit/L RN'ase inhibitor, 1 mmol/L deoxyribonucleoside
triphosphates (dATP, dCTP, dTTP, and dGTP), 2.
Stereoisomeric selectivity of human deoxyribonucleoside