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 (dē-ŏk′sē-rī′bō-no͞o′klē-ə-sīd′, -nyo͞o′-)
A nucleoside that contains deoxyribose as its sugar component.


(diˌɒk sɪˌraɪ boʊˈnu kli əˌsaɪd, -ˈnyu-)

a compound composed of deoxyribose and either a purine or a pyrimidine.
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The PCR reaction was conducted in a 25-[micro]L total volume containing 2.5 (XL of 10 x buffer solution, 2 [micro]L of deoxyribonucleoside triphosphate (dNTP, 2.5 mmol [L.sup.-1]), 1 [micro]L of primer 1 (10 mmol [L.sup.-1]) and primer 2 (10 mmol [L.sup.-1]), 2.5 [micro]L of Mg[Cl.sub.2] (25 mmol [L.sup.-1]), 1 [micro]L of suitable dilution of soil DNA, 2.5 U of Taq DNA polymerase (5 U/[micro]L), and 12.5 [micro]L of double-distilled [H.sub.2]O (dd[H.sub.2]O).
The PCR master mixes consisted of 2.4 [micro]L 5X PCR buffer (Promega, Madison, WI), 1.2 [micro]L 1 mmol [L.sup.-1] deoxyribonucleoside triphosphate, 0.15 [micro]L GoTaq polymerase (Promega, Madison, WI), 1.0 [micro]L 10 [micro]mol [L.sub.-1] bovine serum albumin, 1.2-3.5 [micro]L Mg[Cl.sub.2] (depending on primer), 1.0 [micro]L fluorescently labeled forward primer, 1.0 [micro]L reverse primer, 1.5 [micro]L DNA (5-10 ng [micro][L.sup.-1]), and double-distilled water, to bring the total volume to 12 [micro]L.
PCR was carried out in a volume of 13.0 [micro]L containing 50 ng of genomic DNA, 0.75 [micro]M of each primer (forward: 5'-GAGTCCTTTGTAGGTCCCAAC-3', reverse: 5'-CTTTCTCCCTGTGTCTAGGC-3') as previously designed Ukkola et al., (21) 1 U of Taq DNA polymerase (Thermo Fisher Scientific, Waltham, USA), 3.8 mM of Mg[Cl.sub.2], 10x Mg[Cl.sub.2]-free reaction buffer with [(N[H.sub.4]).sub.2]S[O.sub.4] (Thermo Fisher Scientific, Waltham, MA, USA) and 0.6 mM of deoxyribonucleoside triphosphate mix (Thermo Fisher Scientific, Waltham, USA).
This included 10 ng of genomic DNA, 0.5 [micro]L of 10x PCR Buffer (Sequenom, San Diego, CA, USA), 10 nmol of Mg[Cl.sub.2], 2.5 nmol of deoxyribonucleoside triphosphate (dNTP) Mix (Takara, Dalian, China), 0.5 [micro]mol of Primer Mix, 1U HotStar Taq (Takara, Dalian, China), and 1.8 [micro]L of double-distilled water.
PCR was carried out with 2 [micro]L template DNA, 0.25[micro]M of each primer (F: 5'-GCAAGTCGAGCGGTAGCACAG-3' and R: 5'CAGTGTGGCTGGTCATCCTCTC-3') (260 bp), 0.2 mM deoxyribonucleoside triphosphates, 1x reaction buffer, 2 mM Mg[Cl.sub.2] and 1.5 U Taq DNA polymerase (Fermentas) in a total volume of 25 [micro]L.
PCR reaction (25 [micro]L) was composed of 1x PCR buffer ([Mg.sup.2+]-free), 2mM Mg[Cl.sub.2], 200 [micro]M of each deoxyribonucleoside triphosphate (dNTP), 0.4 [micro]M of each primer, 0.2 U of HotStart Taq DNA polymerase (Takara, Dalian, China), and 2 [micro]L of DNA template.
Briefly, DNA (10 [micro]g) was enzymatically degraded to normal and adducted by deoxyribonucleoside 3'-monophosphates with micrococcal nuclease and spleen phosphodiesterase at pH = 6 and incubated at 37[degrees]C for 3.5 h.
PCR was carried out with 2 [micro]L template DNA, 0.25 of each primer, 0.2 mM deoxyribonucleoside triphosphates, 1x reaction buffer, 2mM Mg[Cl.sub.2] and 0.5 U Prime Taq DNA polymerase (Genet Bio) in a total volume of 25 [micro]L.
The reaction mixture consisted of 5 [micro]L of template DNA, 5 [micro]L 10xPCR buffer (+Mg[Cl.sub.2]) (YeaTaq, Taiwan), 4 [micro]L of deoxyribonucleoside triphosphates (2.5 mmol [L.sup.-1] each of dATP, dTTP, dGTP and dCTP), 0.5 [micro]L of each primer, and 0.25 [micro]L (0.5 U [micro][L.sup.-1]) of Taq DNA polymerase YeaTaq, Taiwan), with 50 [micro]L sterile water added.
Then, 4 [micro]l of reaction buffer, 1 [micro]l of RiboLock RNase inhibitor, 2 [micro]l of deoxyribonucleoside triphosphate, and 1 [micro]l of Moloney Murine Leukemia Virus Reverse Transcriptase were added to the solution until its final volume reached 20 [micro]l.

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