Paraffin slices were dewaxed
and rehydrated, then stained with hematoxylin (Beyotime Biotechnology, China) for 3 min, washed for 5 min, destained with hydrochloric acid alcohol, washed for 5 min, and fast stained with eosin (Beyotime Biotechnology, China).
The tissue was then embedded using paraffin wax, and sections were cut at 4 [micro]m, mounted on a slide, dewaxed
using a hot plate and cleaned in xylene, before hydrating in descending grades of ethanol and, finally, washed in water and stained with Giemsa stain (1:10 dilution) for 30 minutes.
Sections which were formalin fixed and paraffin embedded previously were dewaxed
in xylene, hydrated through decreasing concentrations of ethanol followed by thorough washing in deionised water for 15-20 minutes.
Sections were manually dewaxed
in xylene twice for 5 minutes and brought to distilled water before being treated with 0.
Paraffin sections (4-mm thick) of tissues were dewaxed
and dehydrated in xylene and gradient ethanol, respectively.
Three stains were used on dewaxed
slides: hematoxylin and eosin (H&E), Giemsa, and Martius scarlet blue (MSB) trichrome.
sections were stained with Haemotoxylin and Eosin (H&E).
melanogaster were dewaxed
and dewatered in xylene and ethanol concentration gradient solutions; washed and blocked with 1% BSA at 37degC for 30min avoid light and incubated with caveolin-1 antibody at 4degC for 48h; washed and incubated for 1.
They are then dewaxed
to hydrate the tissue; they are subject to hematoxylin-eosin baths.
The rat hippocampus and cortical were paraffin-embedded, cut into slices, dewaxed
Briefly, the sections were dewaxed
and rehydrated in xylol (Sigma, Germany) and a graded series of 100%, 90%, 80%, and 70% ethanol.
Sections were dewaxed
, rehydrated, and treated for quenching of endogenous peroxidase activity.