diaphorase


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diaphorase

(daɪˈæfəˌreɪs; daɪˈæfəˌreɪz)
n
a flavoprotein enzyme operating in mitochondria, acting as a catalyst in the process of dye reduction or oxidation
References in periodicals archive ?
They used a combination of Cuprolinic blue (which binds to single-stranded RNA) to visualize all neurons and NADPH diaphorase histochemistry, which labels nitrergic neurons (8%-16% of total), almost all of which are situated in the deep submucosa.
Inhibition of diaphorase activity of PfFNR was measured in the reaction of Scheme 1 as described above, by the additions of each alkaloid at 100 ppm, NADPH at 100 uM, and 5 nM FNR.
Then, 50 [micro]l of the next reagent (30 mmol/L nicotinamide, 0.3 [micro]g/ml ADP-ribosyl cyclase, and 100 mmol/L sodium phosphate, pH 8.0) and 100 [micro]l of cycling reagent (100 [micro]g/ml alcohol dehydrogenase, 0.1 mg/ml BSA, 2% ethanol, 20 [micro]mol/L resazurin, 10 [micro]mol/L of FMN, 10 [micro]g/ml diaphorase, 10 mmol/L nicotinamide, and 100 mmol/L sodium phosphate, pH 8.0) were sequentially added to 100 [micro]l of each samples and incubated for 15 min and 4 h, respectively, at room temperature.
The final assay mixture comprised 110 mM lactate, 3.35 mM [NAD.sup.+], 350 [micro]M resazurin, and 2.2 units/mL of diaphorase in 3 mM Tris/30 mM HEPES/10 mM NaCl buffer (pH 7.4) and 0.45% Triton X-100.
The red cell nicotinamide adenine dinucleotide hydrogen (NADH) dependent methaemoglobin reductase or diaphorase I enzyme (Breaking et al., 1951; Gibson and Harrison, 1947), protects the erythrocyte from an accumulation of methaemoglobin.
Quercetin actively participates in intracellular signaling, inhibiting phosphatidylinositol-3 kinase, protein kinase C, xanthine oxidase and NADPH diaphorase (Gschwendt et al.
Keywards: Doxorubicin, Oxidative stress ,Cardiotoxicity, Simvastatin, Antioxidant enzymes, DT diaphorase.
Mitochondrial enzymes, as carriers of diaphorase activities, like NADPH dehydrogenase, are probably responsible for the transference of electrons from NADPH + [H.sup.+] to resazurin, which is reduced to resorufin.
NADH-dependent oxidoreductase (diaphorase) activity and isozyme pattern of sperm in infertile men.
RH1's activity is enhanced by an enzyme, DT diaphorase, which often is found in cancers of the lungs, liver, and breasts.