They used a combination of Cuprolinic blue (which binds to single-stranded RNA) to visualize all neurons and NADPH diaphorase
histochemistry, which labels nitrergic neurons (8%-16% of total), almost all of which are situated in the deep submucosa.
Inhibition of diaphorase
activity of PfFNR was measured in the reaction of Scheme 1 as described above, by the additions of each alkaloid at 100 ppm, NADPH at 100 uM, and 5 nM FNR.
Then, 50 [micro]l of the next reagent (30 mmol/L nicotinamide, 0.3 [micro]g/ml ADP-ribosyl cyclase, and 100 mmol/L sodium phosphate, pH 8.0) and 100 [micro]l of cycling reagent (100 [micro]g/ml alcohol dehydrogenase, 0.1 mg/ml BSA, 2% ethanol, 20 [micro]mol/L resazurin, 10 [micro]mol/L of FMN, 10 [micro]g/ml diaphorase
, 10 mmol/L nicotinamide, and 100 mmol/L sodium phosphate, pH 8.0) were sequentially added to 100 [micro]l of each samples and incubated for 15 min and 4 h, respectively, at room temperature.
The final assay mixture comprised 110 mM lactate, 3.35 mM [NAD.sup.+], 350 [micro]M resazurin, and 2.2 units/mL of diaphorase
in 3 mM Tris/30 mM HEPES/10 mM NaCl buffer (pH 7.4) and 0.45% Triton X-100.
The red cell nicotinamide adenine dinucleotide hydrogen (NADH) dependent methaemoglobin reductase or diaphorase
I enzyme (Breaking et al., 1951; Gibson and Harrison, 1947), protects the erythrocyte from an accumulation of methaemoglobin.
Quercetin actively participates in intracellular signaling, inhibiting phosphatidylinositol-3 kinase, protein kinase C, xanthine oxidase and NADPH diaphorase
(Gschwendt et al.
reported 3 brothers with congenital recessive methemoglobinemia due to homozygous NADH diaphorase
Keywards: Doxorubicin, Oxidative stress ,Cardiotoxicity, Simvastatin, Antioxidant enzymes, DT diaphorase
Mitochondrial enzymes, as carriers of diaphorase
activities, like NADPH dehydrogenase, are probably responsible for the transference of electrons from NADPH + [H.sup.+] to resazurin, which is reduced to resorufin.
NADH-dependent oxidoreductase (diaphorase
) activity and isozyme pattern of sperm in infertile men.
RH1's activity is enhanced by an enzyme, DT diaphorase
, which often is found in cancers of the lungs, liver, and breasts.