As shown in Figure 1, real-time RT-PCR analysis showed that the expression of LIF in the endometrium increased at late
diestrus and proestrus phases during the estrous cycle (linear effect of day, p<0.05), while the expression of LIFR did not change during the cycle.
Female mice were observed to run more immediately after the pro-estrus phase (7.8 km per day) and less after the
diestrus (5.5 km per day) (29).
Smears were scored as follows: proestrus (P) contained primarily nucleated epithelial cells with no or few cornified cells, estrus (E) consisted of mostly cornified cells, metestrus (M) contained epithelial cells with leucocyte attacks, and
diestrus (D) consisted of few cells that were leucocytes with some rounded or cornified epithelial cells.
Our previous study confirmed a significant lengthening of the estrous cycle and the
diestrus phase, and an increase in the progesterone concentration in female rats treated with dill extracts, but the stereological study did not reveal any changes in the volumes of ovaries, and the primary, secondary, and graafian follicles (3).
Diestrus. This stage depicted a predominance of neutrophils as shown with methylene blue stain (Fig.
Vaginal Exfoliative Cytology: The smears revealed predominant numbers of intermediate cells and neutrophils suggestive of
diestrus stage of estrous cycle and uterine infection.
Response and fertility of dairy heifers following injection with prostaglandin F2[alfa] during early, middle, and late
diestrus. Theriogenology 23: 655-661.
Most cervical lacerations are best evaluated in
diestrus, as the increased cervical tone facilitates assessment of the apposition of cervical folds and damage to the muscular layer.
The estrus cycle of mice is approximately 4-5 days, including proestrus, estrus, metestrus and
diestrus (15).
Based on vaginal cytology, females were divided into two groups: diestrous (those in
diestrus and metaestrus) and estrous (those in estrus and proestrus).
The estrous cycle comprises four stages: estrus,
diestrus 1,
diestrus 2, and proestus.
We generated cDCs in hormone-depleted medium supplemented with 17-[beta]-estradiol (E2) at doses comparable to those present in the serum of male mice (0.03 nanomolar (nM)), in the serum of female mice in
diestrus, or in a regular fetal bovine serum (0.1 nM) [51, 52].
