The genes were detected with three anchored primers and ten random primers using differential display
reverse transcription PCR (DDRT-PCR).
In 1992, Liang and Pardee  developed a new PCR-based technique called Differential Display
(DDRTPCR), which was useful for comparing and identifying changes in gene expression at the mRNA level among different eukaryotic cell samples.
Moreover, the differential display
can simultaneously visualize increased or decreased expression of numerous mRNAs from many samples, and requires relatively small amounts of starting material (Pardee and McClelland, 1999).
As if taking a cue from the flamboyance of the bold yachtsmen who will sail the AC72, the French fashion house Louis Vuitton has created a watch, the Tambour Twin Chrono, a single- push bi- chronograph with differential display
, to commemorate the 30th Anniversary of the LV Cup.
RT-PCR was performed on salt sensitive line of sunflower plants to investigate expression pattern of related transcripts under salt-stress.
In the present investigation of the differential display
of serum proteomes before and after a 12-week TCC exercise program, we have demonstrated and validated a unique proteomic profile of TCC exercise.
We have identified a set of genes differentially expressed in livers between these two breeds by the mRNA differential display
technique (DDRT-PCR) which was first reported by Liang and Pardee (1992) as a fast and efficient method for investigating differences in gene expression.
Application of differential display
RT-PCR to the analysis of gene expression in a plant-fungus interaction.
In order to determine if other metabolic processes could be important in the metabolism of glyphosate in plants and could contribute to resistance to glyphosate, we used differential display
techniques to compare genes expressed in Eleusine indica resistant and susceptible to glyphosate.
Content covers such topics as differential display
, electrophoresis, genotyping, transgenic systems, mutation screens, protein spectrometry, comparative genomics and phage display.
Identification and cloning of new cadmium-responsive genes by differential display
After the three days of treatment, a differential display
technique was used to identify the genes that had been regulated in the treated scallops, but not in the controls.